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目的建立同时测定双五胶囊中紫丁香苷、五味子醇甲、五味子甲素、五味子乙素含量的方法。方法HPLC法,采用Diamonsil C18柱(200mm×4.6mm,5μm),流动相为A:甲醇-乙腈(1∶1),B:水,梯度洗脱,0～5min,35%～60%A;5～10min,60%～70%A;10～50min,70%～90%A;50～90min,90%A。流速为1ml/min,柱温:35℃,检测波长220nm。结果紫丁香苷、五味子醇甲、五味子甲素、五味子乙素浓度分别在1.28～20.40μg/ml(r=0.9997)、6.30～100.80μg/ml(r=0.9996)、1.20～19.20μg/ml(r=0.9998)和3.75～60.00μg/ml(r=0.9996)范围内呈良好的线性关系。4种成分精密度实验RSD<1%。24h内稳定性RSD<1.5%。紫丁香苷、五味子醇甲、五味子甲素、五味子乙素的平均加样回收率分别为99.47%、102.50%、99.21%、101.86%。结论所建立的方法具有快速、简便、准确等优点,可用于双五胶囊的质量控制。
Objective To establish a method for the simultaneous determination of syringin, schisandrin, schisandrin and schisandrin in Shuangwu Capsules. Methods HPLC was carried out on a Diamonsil C18 column (200 mm × 4.6 mm, 5 μm) with a mobile phase of A: methanol - acetonitrile (1: 1), gradient elution with B: 5 to 10 min, 60% to 70% A, 10 to 50 min, 70% to 90% A, 50 to 90 min, 90% Flow rate was 1ml / min, column temperature: 35 ℃, detection wavelength 220nm. Results The results showed that the concentrations of syringin, schisandrin, schisandrin and schizandrin were 1.28-20.40μg / ml (r = 0.9997), 6.30-100.80μg / ml (r = 0.9996), 1.20-19.19μg / ml r = 0.9998) and 3.75 ~ 60.00μg / ml (r = 0.9996). RSD <1% of 4 components precision experiment. 24h stability RSD <1.5%. The average recoveries of syringin, schisandrin, schisandrin and schisandrin B were 99.47%, 102.50%, 99.21% and 101.86%, respectively. Conclusion The established method has the advantages of fast, simple and accurate, and can be used for the quality control of Shuangwu capsule.