Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers

来源 :Asian Pacific Journal of Tropical Biomedicine | 被引量 : 0次 | 上传用户:ppsl21
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Objective:To evaluate and compare the antioxidant potential and anti-inflammatory activity of ethanolic extract of flowers of Moringa oleifera(M.oleifera)grown in Oman.Methods:Flowers of M.oleiferawere collected in the month of December 2012 and identified by a botanist.Alcoholic extract of the dry pulverized flowers ofM.oleiferawere obtained by cold maceration method.The ethanolic flower extract was subjected to preliminary phytochemical screening as the reported methods.Folin-Ciocalteu reagent was used to estimate total phenolic content.DPPH was used to determinein-vitroantioxidant activity and anti-inflammatory activity of flowers was investigated by protein denaturation method.Results:Phytochemical analysis of extract showed presence of major classes of phytochemicals such as tannins,alkaloids,flavonoids,cardiac glycosidesetc.M.oleiferaflowers were found to contain 19.31 mg/g of gallic acid equivalent of total phenolics in dry extract but exhibited moderate antioxidant activity.The anti-inflammatory activity of plant extract was significant and comparable with the standard drug diclofenac sodium.Conclusions:The results of our study suggest that flowers ofM.oleiferapossess potent anti-inflammatory activity and are also a good source of natural antioxidants.Further study is needed to identify the chemical compounds responsible for their anti-inflammatory activity. Objective: To evaluate and compare the antioxidant potential and anti-inflammatory activity of ethanolic extract of flowers of Moringa oleifera (M. oleifera) grown in Oman. Methods: Flowers of M. oleiferawere collected in the month of December 2012 and identified by a botanist . Alcoholic extracts of the dry pulverized flowers of M. oleiferawere obtained by cold maceration method. The ethanolic flower extract was subjected to preliminary phytochemical screening as the reported methods. Folin-Ciocalteu reagent was used to estimate total phenolic content. DPPH was used to determinein- vitroantioxidant activity and anti-inflammatory activity of flowers was investigated by protein denaturation method. Results: Phytochemical analysis of extract exhibited presence of major classes of phytochemicals such as tannins, alkaloids, flavonoids, cardiac glycoside settings. M. oleifera flowers were found to contain 19.31 mg / g of gallic acid equivalent of total phenolics in dry extract but exhibited moderate antioxidant activity.The anti-inflammatory activity of plant extract was significant and comparable with the standard drug diclofenac sodium. Conclusions: The results of our study suggest that that flowers of M. oleiferapossess potent anti-inflammatory activity and are also a good source of natural antioxidants. Future study is needed to identify the chemical compounds responsible for their anti-inflammatory activity.
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