论文部分内容阅读
目的筛选大孔树脂分离纯化菊苣香豆素的最佳工艺。方法以香豆素的吸附量为指标,采用单因素试验法对影响菊苣提取物纯化效率的因素进行优化,分别采用UV和HPLC测定纯化后固形物中总香豆素和秦皮乙素的浓度。结果最佳洗脱程序为,选定D101大孔树脂,上样浓度为0.35 mg.mL 1,最大上样量为35 BV(35倍柱体积),上样速度为0.067BV.min 1,依次用11 BV的蒸馏水以0.067 BV.min 1的流速洗脱,9 BV的30%乙醇以0.033 BV.min 1的流速洗脱,弃去洗脱液,再用9 BV的80%乙醇以0.033 BV.min 1的流速洗脱,收集洗脱液。纯化得到的固形物中菊苣香豆素含量为47.00%,秦皮乙素含量为6.38%,香豆素的转移率为81.74%。结论纯化工艺简便、稳定,能提高菊苣中活性组分的含量。
Objective To screen the best technology of separation and purification of chicory coumarin by macroporous resin. Methods The coumarin adsorption capacity was used as an index to optimize the factors influencing the purification efficiency of chicory extract by single factor test. The concentrations of total coumarins and aesculetin in the solid samples were determined by UV and HPLC respectively. Results The optimal elution program was as follows: D101 macroporous resin was selected, the sample concentration was 0.35 mg.mL 1, the maximum sample volume was 35 BV (35 column volumes), the loading speed was 0.067 BV.min 1, Elute with 11 BV of distilled water at a flow rate of 0.067 BV · min 1 and 9 BV of 30% ethanol at a flow rate of 0.033 BV · min 1, discard the eluate and rehydrate with 9 BV of 80% ethanol at 0.033 BV .min 1 flow rate elution, collecting eluent. The purified solid chicory coumarin content of 47.00%, aesculetin B content of 6.38%, coumarin transfer rate was 81.74%. Conclusion The purification process is simple and stable, and can increase the content of active components in chicory.