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目的:本研究为观察rhlL-6对小鼠造血功能的影响。方法:应用致死性(?)Co-Y射线辐照BALB/c小鼠,造成骨髓型放射损伤模型。在照射后用rhIL-6皮内注射给药6天(2pg/Bid/只),通过检测出、凝血时间(BT、CT)、凝血酶原时间(PT)、外周血PLT和WBC,以及骨髓造血细胞体外培养CFU-Mix和脾脏CFU-S的数目,研究rhIL-6对骨髓型放射损伤小鼠早期造血功能恢复的效应,并进行机理的探讨。结果:rhIL-6处理组小鼠的BT、CT和PT均比对照组显著缩短(P<0.01),外周血PLT和WBC分别比对照组提高130％和165％,促进造血干/祖细胞(HSC/HPC)增殖、分化。骨髓造血细胞体外培养CFU-Mix和脾脏CFU-S计数均显著高于对照组(P<0.01)。结论:以上说明rhIL-6具有促进造血干/祖细胞(HSC/HPC)增殖,分化有利于受照小鼠早期造血细胞恢复的生物效应,可减轻辐射所致的骨髓造血功能的损伤。
Objective: To investigate the effect of rhlL-6 on hematopoietic function in mice. Methods: BALB / c mice were irradiated by lethal (?) Co-gamma rays, resulting in a bone marrow-derived radiation injury model. After irradiation, rhIL-6 was intradermally injected for 6 days (2 pg / bid / only) and clotting time (BT, CT), prothrombin time (PT), peripheral blood PLT and WBC, and bone marrow To investigate the effect of rhIL-6 on the recovery of hematopoietic function in mice with radiation-injured bone marrow after CFU-Mix and spleen CFU-S cultured in vitro, and to explore the mechanism. Results: The levels of BT, CT and PT in rhIL-6-treated mice were significantly shorter than those in control group (P <0.01). The levels of PLT and WBC in peripheral blood were increased by 130% and 165% HSC / HPC) proliferation and differentiation. The counts of CFU-Mix and spleen CFU-S in bone marrow hematopoietic cells cultured in vitro were significantly higher than those in the control group (P <0.01). Conclusion: The above shows that rhIL-6 has the biological effect of promoting the proliferation and differentiation of hematopoietic stem / progenitor cells (HSC / HPC) in the recovery of hematopoietic cells in irradiated mice, and can lessen the damage of hematopoietic function caused by radiation.