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选取HCV－1、HCV－J、HCV－BK、台湾株HCV、中国河北株HCV序列的同源性部分，设计Ns3区部分序列的PCR引物．应用RT－PCR技术，从丙型肝炎患者血浆中扩增一长为448bp的cDNA片段作为目的基因，与经过限制性酶切的pUcI9载体连接，构建重组质粒pUHCV－Ns3．分别或混合应用HCV序列特异的引物和载作序列特异的通用引物，以PCR扩增重组质粒，结果扩增产物的分子量均与预期大小一致．限制性酶切位点分析结果也证实，克隆的基因是HCV的Ns3区部分序列的目的基因．克隆序列的特异性和插入方向同时得到了鉴定．
Homologous part of HCV sequences of HCV-1, HCV-J, HCV-BK, Taiwan strain HCV and Hebei strain of China were selected and PCR primers of part of Ns3 region were designed. A 448 bp cDNA fragment was amplified from the plasma of hepatitis C patients by RT-PCR. The recombinant plasmid pUHCV-Ns3 was constructed by ligating it with pUcI9 vector. The PCR products were amplified by PCR. The results showed that the molecular weight of the amplified product was consistent with the expected size. The results of restriction analysis also confirmed that the cloned gene was the target gene of Ns3 region partial sequence of HCV. The specificity and insertion orientation of the cloned sequences were identified at the same time.