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目的揭示脑老化神经递质受体变化的性质，受体蛋白质遗传信息的改变与受体变化的关系。方法采用异硫氰酸胍－苯酚－氯仿法和美国Ｐｒｏｍｅｇａ新磁珠技术，自大鼠脑组织中提取多聚嘌呤ｍＲＮＡ〔Ｐｏｌｙ（Ａ）＋ｍＲＮＡ〕，注入非洲爪蟾卵母细胞内，用电压箝位法检测表达到卵母细胞上的递质受体的电学反应。结果注射老年大鼠脑ｍＲＮＡ的卵母细胞对４种递质——乙酰胆碱、谷氨酸、多巴胺和γ－氨基丁酸诱发的电流幅度下降，分别为成年对照组的４３％、５８％、４１％和４５％，与成年对照组比较差异均有显著性。乙酰胆碱受体电流的逆转电位约－２０ｍＶ，Ｃｌ－是该电流的主要载流离子。结论老年大鼠脑各类型的递质受体功能均呈减弱趋势，其原因可能是受体蛋白遗传信息发生了改变
Objective To reveal the nature of neurotransmitter receptor changes in brain aging, the relationship between genetic alterations of receptor proteins and receptor changes. Methods Polyglutamate mRNA (Poly (A) + mRNA] was extracted from rat brain tissue by guanidine isothiocyanate - phenol - chloroform method and Promega magnetic beads technology. The poly (A) mRNA was injected into Xenopus laevis oocytes, Clamp method to detect the electrical response of the transmitter receptor expressed on the oocyte. Results The current amplitude induced by 4 neurotransmitters - acetylcholine, glutamate, dopamine and γ-aminobutyric acid was decreased in oocytes injected with brain mRNA in aged rats, which were 43%, 58%, 41% % And 45%, respectively, compared with the adult control group, the difference was significant. The reversal potential of the acetylcholine receptor current is about -20 mV, and Cl- is the main carrier current for this current. Conclusion The function of various types of neurotransmitter receptors in the rat brain showed a tendency to weaken, which may be due to the change of the genetic information of the receptor protein