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本文观察了大鼠黑质内注射6-羟多巴胺(6-OH-DA)对电针镇痛作用的影响及荧光组织化学变化。用对尾部皮肤等速增加的直流电流为疼痛刺激,测定引起甩尾和嘶叫所需要的电流强度做为基础痛阈值。测基础痛阈后电针30分钟,从电针开始10分钟后,每隔10分钟测痛一次,共5次。然后,将动物分为两组:实验组黑质内注6-OH-DA(4μg/2μl),对照组仅注配药液。饲养7天后再测痛一次。最后,按Faglu法灌流、取脑,做连续冰冻切片进行荧光组织化学检查。结果显示实验组电针镇痛作用明显增强,电针开始后所测得的5次痛阈值均在160％以上,与注药前相比差异非常显著(P<0.01)。同时,黑质内发黄绿色荧光的DA神经元部分消失。而注配药液组,电针镇痛作用和荧光组织化学均无明显变化。结果表明黑质内注6-OH-DA损毁DA神经元有加强电针镇痛并使持续的时间延长。这进一步提示黑质一纹体DA通路可能具有对抗电针镇痛的作用。许多研究表明脑内多巴胺(DA)能神经元系统与针刺镇痛有关。家兔尾核内注DA受体阻断剂氟哌啶醇可增强电针的镇痛作用,注入DA则抑制之。而黑质注DA能增强针效。这提示黑质纹状体DA系统可能具有对抗电针镇痛的作用。但也有人提出脑内DA能系统与针刺镇痛有协同作用。本文用特异性细胞毒性物质6-羟多巴胺(6-OH-DA)损毁黑质的DA能通路,进一步观察黑质DA能神经元系统在电针镇痛中的作用。
In this paper, the effect of intrasular injection of 6-OHDA on the analgesic effect of electroacupuncture and the change of histochemical organization were observed. Using a DC current at an isokinetic increase in the tail skin as a pain stimulus, the current intensity required to cause flicking and barking was determined as the basal pain threshold. After the basic pain threshold was measured, the electroacupuncture was performed for 30 minutes. After 10 minutes from the start of the electroacupuncture, the pain was measured every 10 minutes for a total of 5 times. Then, the animals were divided into two groups: 6-OH-DA (4 μg/2 μl) was injected intraperitoneally into the experimental group, while the control group was only injected with the drug solution. After 7 days of feeding, the pain was measured again. Finally, the brain was perfused with Faglu, and frozen sections were taken for fluorescent histochemical examination. The results showed that the analgesic effect of electroacupuncture in the experimental group was significantly enhanced, and the five pain thresholds measured after the onset of electroacupuncture were all above 160%, and the difference was very significant before injection (P<0.01). At the same time, DA neurons that had yellow-green fluorescence in the substantia nigra disappeared. There was no significant change in the analgesic effect and fluorescence histochemistry of the electroacupuncture group. The results showed that destruction of DA neurons by intraperitoneal injection of 6-OH-DA enhanced electroacupuncture analgesia and prolonged duration. This further suggests that the striatal DA pathway may have an anti-electroacupuncture analgesic effect. Many studies have shown that the intracerebral dopamine (DA)ergic neuronal system is associated with acupuncture analgesia. Administration of the DA receptor blocker haloperidol in the rabbit tail nucleus enhanced the analgesic effect of electroacupuncture and was inhibited by DA injection. The DA quality DA can enhance the needle effect. This suggests that the nigrostriatal DA system may have an anti-electroacupuncture analgesic effect. However, it has also been suggested that the brain DA system has a synergistic effect with acupuncture analgesia. In this article, we use the specific cytotoxic substance 6-hydroxydopamine (6-OH-DA) to damage the DA energy pathway of the substantia nigra, and further observe the role of DA neurons in the substantia nigra in electroacupuncture analgesia.