论文部分内容阅读
本研究观察了二氧化氮（ＮＯ２）对大鼠肺脂质过氧化、抗氧化酶及花生四烯酸代谢的影响。实验选用Ｗｉｓｔａｒ雄性健康成年大鼠，体重１５０～１７５ｇ，设计浓度：自然空气，０．１５，１．５，１５ｍｇ／ｍ３ＮＯ２组。暴露方法：静式染毒，每天２小时，每周６天，染毒４周。实验结果表明：（１）随着ＮＯ２浓度的递增，大鼠尿中血栓烷素Ｂ２与６－酮－前列腺素Ｆ１α比值逐渐升高，且存在剂量－反应关系。（２）１．５ｍｇ／ｍ３ＮＯ２能致大鼠肺丙二醛含量增加，同时可见谷胱甘肽过氧化物酶活性升高，且两者相关具有高度显著性（Ｐ＜０．０１）。这表明：大鼠肺能通过某些抗氧化酶活性的升高而免受脂质过氧化物的毒害。（３）１．５ｍｇ／ｍ３ＮＯ２能致大鼠肺血栓烷素Ｂ２增加，６－酮－前列腺素Ｆ１α降低。同时可见丙二醛与６－酮－前列腺素Ｆ１α之间存在显著负相关（Ｐ＜０．０５）。经对尿中血栓烷素Ｂ２，６－酮－前列腺素Ｆ１α及其比值与肺中相应指标作相关分析，各指标相关具有显著性。表明：测定尿中血栓烷素Ｂ２与６－酮－前列腺素Ｆ１α含量的变化能反映ＮＯ２对大鼠肺花生四烯酸代谢的影响，用它作为一个能反映ＮＯ２健康效应、灵敏的生化指标成为可能。
This study investigated the effects of nitric oxide (NO2) on lipid peroxidation, antioxidant enzymes and arachidonic acid metabolism in rat lungs. Experimental selection Wistar male healthy adult rats, weighing 150 ~ 175g, design concentration: natural air, 0.15,1.5,15 mg / m3NO2 group. Methods of exposure: Static exposure, 2 hours a day, 6 days a week, for 4 weeks. The experimental results showed that: (1) As the concentration of NO2 increased, the ratio of urinary thromboxane B2 to 6-keto-PGF1α gradually increased, and there was a dose-response relationship. (2) 1.5mg / m3NO2 could increase the content of MDA in rat lungs, and at the same time, the glutathione peroxidase activity increased, and the correlation between them was highly significant (P <0.01). This suggests that rat lungs are protected from lipid peroxides by the elevation of certain antioxidant enzymes. (3) 1.5mg / m3NO2 can cause rat thromboxane B2 increased, 6-keto-prostaglandin F1α decreased. At the same time, there was a significant negative correlation between MDA and 6-keto-PGF1α (P <0.05). After urinary thromboxane B2,6-keto-prostaglandin F1α and its ratio and the corresponding indicators in the lung for the correlation analysis, the correlation was significant. The results showed that the change of urinary content of thromboxane B2 and 6-keto-prostaglandin F1α could reflect the effect of NO2 on the metabolism of arachidonic acid in rat lung. Using it as a sensitive biochemical indicator that can reflect the health effect of NO2, may.