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本文利用离体大鼠等容收缩心脏灌流模型观察了不同的pH值再灌液对缺血—再灌注损伤的影响。结果显示:心脏旷置30min后,先用pH6.8的HEPES—KH液体复灌7min,然后换成pH7.1的液体灌流7min,最后恢复pH7.4的液体灌流6min,心脏各血液动力学指标均优于单纯用pH7.4液体复灌组,再灌期最大左室舒张末压降低,±dp/dt升高,与缺血前相比心脏功能指标恢复百分率上升,尤其可以明显减轻再灌注后3～5min之内的心脏挛缩。另外,再灌期冠脉流出液中LDH含量减少。提示:pH反常是心肌缺血—再灌注损伤的重要发病机制之一,复灌的初始用酸性液体可以减轻pH反常引起的心脏舒缩功能障碍。
In this paper, isolated rat isovolumic contractile cardiac perfusion model was used to observe the effect of different pH reperfusion fluid on ischemia-reperfusion injury. The results showed that: 30min after exclusion of heart, HEPES-KH solution with pH6.8 was used for reperfusion for 7min, and then the solution was changed to pH7.1 for 7min. Finally, the solution was resuspended for 6min at pH7.4. The hemodynamics Were better than that of the liquid reperfusion group treated with pH7.4 alone. The maximal left ventricular end-diastolic pressure and the increase of ± dp / dt during reperfusion were significantly higher than those before reperfusion After 3 ~ 5min of the heart contracture. In addition, LDH content in coronary effluent decreased after reperfusion. Tip: pH abnormality is one of the important pathogenesis of myocardial ischemia-reperfusion injury. The initial rehydration with acidic liquid can relieve cardiac dysfunction caused by pH abnormality.