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目的 比较 p6 3及 p5 3、E cadherin(E cad)、Ki 6 7在肺癌中的表达 ,以了解在不同组织类型肺癌发生发展过程中 ,p6 3与抑癌基因 (p5 3)突变、上皮分化标志基因 (E cad)失活及细胞增殖标志基因 (Ki 6 7)激活有无相关性。方法 采用免疫组化S P法分别检测 6 1例原发性肺癌中 p6 3、p5 3、E cad和Ki 6 7的表达情况。 结果 p6 3在肺鳞癌中阳性率为 10 0 0 % ,而在其他组织类型肺癌中 p6 3基本不表达 ,差异有显著性 (P <0 0 5 ) ;在不同分化程度的肺鳞癌中 p6 3、p5 3的表达差异有显著性 (P<0 0 5 ) ,E cad、Ki 6 7的表达差异无显著性 (P >0 0 5 ) ;E cad的表达在小细胞肺癌与肺鳞癌和肺腺癌之间差异有显著性 (P <0 0 5 ) ;Ki 6 7的表达在各种组织类型肺癌之间差异有显著性 (P <0 0 5 ) ;在不同分化程度鳞癌中 p6 3与E cad的表达呈负相关(P <0 0 5 )。结论 p6 3可作为鳞状上皮源性肿瘤标记物 ,是判断鳞状细胞癌的增殖和分化有意义的指标 ,并可作为鉴别分化差的鳞癌和腺癌、小细胞癌的指标。
Objective To compare the expression of p6 3 and p5 3, E cadherin (E cad) and Ki 6 7 in lung cancer and to find out the relationship between the expression of p6 3 and tumor suppressor gene (p5 3), epithelial differentiation E cad inactivation and cell proliferation marker gene (Ki 6 7) activation of whether the correlation. Methods The expressions of p6 3, p5 3, E cad and Ki 6 7 in 61 primary lung cancer specimens were detected by immunohistochemical SP method. Results The positive rate of p63 in lung squamous cell carcinoma was 100%, while p6 3 was not expressed in other tissues of lung cancer (P <0.05). In differentiated lung squamous cell carcinoma p6 3, p5 3, but there was no difference between E cad and Ki 6 7 (P> 0.05). The expression of E cad in small cell lung cancer and lung squamous cell carcinoma (P <0.05). The expression of Ki 6 7 was significantly different between various types of lung cancer (P <0.05). In different degrees of differentiation of squamous cell carcinoma There was a negative correlation between p6 3 and E cad expression (P <0.05). Conclusion p6 3 can be used as a marker of squamous epithelium-derived tumor, which is a useful marker to judge the proliferation and differentiation of squamous cell carcinoma. It can be used as an index to differentiate squamous cell carcinoma, adenocarcinoma and small cell carcinoma.