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目的比较ＣｏｕｒｔｅｎａｙＭｉｌｓ克隆测定法和生长测定法对放射处理后活存肿瘤细胞的检测效果。方法选用人类乳腺癌ＭＣＦ７及结肠癌Ｃｏｌｏ２０５细胞株，在接受２Ｇｙ、４Ｇｙ、８Ｇｙ放射处理后，用以上两种方法检测活存的肿瘤细胞数。结果在克隆测定组，被检测的活存细胞均具有克隆能力；Ｃｏｌｏ２０５悬浮生长和贴壁生长细胞对放射线的敏感性没有差别。在生长测定组，“相对活存指数”于培养后第６～９天为最小，其平均值接近于克隆法。结论生长测定可以为细胞增殖的早期活动状态提供丰富的信息，而Ｃｏｕｒｔｅｎａｙ克隆测定则确切地反映了具有克隆能力的活存细胞数，并且适用于所有生长形式的细胞。
Objective To compare the detection effects of Courtenay-Mils cloning assay and growth assay on the survival of tumor cells after radiation treatment. METHODS: Human breast cancer MCF-7 and colon cancer cell line Colo205 were selected. After receiving 2 Gy, 4 Gy, and 8 Gy of radiation treatment, the number of living tumor cells was measured by the above two methods. Results In the cloning assay group, all viable cells tested had clonality; there was no difference in the sensitivity of Colo205 suspension growth and adherent growth cells to radiation. In the growth assay group, the “relative viability index” was the smallest from day 6 to day 9 after culture, and the average value was close to the cloning method. Conclusions Growth assays can provide a wealth of information on the early activity of cell proliferation, while Courtenay cloning assays accurately reflect the number of viable cells with clonality and are applicable to all cell types that grow.