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窖蛋白-1(caveolin-1)是胞膜窖(caveolae)中重要的结构和功能蛋白.Caveolin-1参与细胞的多种生命活动并与恶性肿瘤的发生相关.为探讨caveolin-1对胰腺癌细胞PANC1的体外增殖、迁移、侵袭以及裸鼠体内成瘤能力的影响,通过基因转染技术培育caveolin-1过表达细胞株PANC1/cav-1作为实验组,转染空载体细胞株PANC1/vector作为对照组,采用RT-PCR及Western blot方法检测caveolin-1的表达量,流式细胞术分析细胞周期,软琼脂细胞克隆实验检测细胞增殖能力,侵袭小室实验检测癌细胞迁移和侵袭的能力,建立裸鼠皮下种植瘤模型并检测肿瘤组织的增殖与凋亡.PANC1/cav-1中的caveolin-1表达稳定,表达量明显高于对照组细胞株和亲本细胞株(P<0.01),细胞周期检测显示大量PANC1/cav-1细胞被抑制于G0/G1期,caveolin-1抑制PANC1的增殖,迁移和侵袭能力.在裸鼠的体内实验中,caveolin-1显著抑制PANC1细胞在裸鼠体内的生长,Ki-67染色和TUNEL染色表明在PANC1细胞中过表达caveolin-1,可以抑制肿瘤增殖并诱导肿瘤凋亡.上述结果表明,caveolin-1可能通过对胰腺癌细胞周期的影响(抑制于G0/G1期),抑制胰腺癌PANC1细胞在体内外的增殖、迁移和侵袭,并导致肿瘤凋亡.
Caveolin-1 is an important structural and functional protein in the caveolae.Caveolin-1 is involved in various cell life activities and is associated with the development of malignant tumors.To investigate the effect of caveolin-1 on pancreatic cancer Cell proliferation, migration and invasion of PANC1 in vitro and tumorigenicity in nude mice. PANC1 / cav-1 overexpressing caveolin-1 was transfected into PANC1 / vector by using gene transfection technique. As the control group, the expression of caveolin-1 was detected by RT-PCR and Western blot. The cell cycle was analyzed by flow cytometry. The ability of cell proliferation was detected by soft agar cell cloning assay. The model of subcutaneously implanted tumor in nude mice was established and the proliferation and apoptosis of tumor tissue were detected.The expression of caveolin-1 in PANC1 / cav-1 was stable and the expression level was significantly higher than that in control group and parental cell line (P <0.01) Cyclic detection showed that a large number of PANC1 / cav-1 cells were inhibited in G0 / G1 phase and caveolin-1 inhibited the proliferation, migration and invasion of PANC1.In the in vivo experiments, caveolin-1 significantly inhibited PANC1 cells in nude mice Growth, The results of Ki-67 staining and TUNEL staining indicated that caveolin-1 overexpression caveolin-1 can inhibit tumor proliferation and induce apoptosis in PANC1 cells.The above results suggest that caveolin-1 may affect the cell cycle of pancreatic cancer by inhibition of G0 / G1 Stage), inhibit pancreatic cancer PANC1 cells in vitro and in vivo proliferation, migration and invasion, and lead to tumor apoptosis.