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目的:研究维通注射剂(Weitong injection,WTI)对大鼠腹膜正常成纤维细胞(normal fibroblast,NFB)与粘连成纤维细胞(adhesive fibroblast,AFB)增殖活性与细胞周期的影响。方法:利用原代培养获得大鼠NFB与AFB,以地塞米松为阳性对照药,采用MTT法和流式细胞术考察WTI对2种成纤维细胞(fibro-blast,FB)增殖活性与细胞周期的影响。结果:WTI对AFB的半数抑制浓度(50%inhibitory concentration,IC50)显著低于NFB(P<0.01),WTI对2种FB的IC50均显著低于地塞米松(P<0.01);WTI高、中剂量组和地塞米松组的AFB G0/G1期比例显著高于AFB空白对照组(P<0.01),S期比例显著低于AFB空白对照组(P<0.01);WTI高、中剂量组AFB的G0/G1期比例显著高于地塞米松组(P<0.01);各药物组的NFB细胞周期与空白对照组相比无显著性差异。结论:WTI通过将AFB停滞于G0与G1期,延缓其进入S期,抑制AFB增殖活性,WTI对AFB的作用强于NFB。
Objective: To investigate the effect of Weitong injection (WTI) on the proliferation and cell cycle of normal fibroblast (NFB) and adhesive fibroblast (AFB) in rats. Methods: NFB and AFB were obtained by primary culture. Dexamethasone was used as a positive control. MTT assay and flow cytometry were used to investigate the effect of WTI on the proliferation and fibroblast proliferation of two fibroblasts (FB) Impact. Results: The IC50 of WTI to AFB was significantly lower than that of NFB (P <0.01), and the IC50 of WTI to both FB was significantly lower than that of dexamethasone (P <0.01) The ratio of AFB G0 / G1 phase in middle dose group and dexamethasone group was significantly higher than that in AFB blank control group (P <0.01), and the proportion in S phase was significantly lower than that in AFB blank control group (P <0.01) The ratio of G0 / G1 phase in AFB group was significantly higher than that in dexamethasone group (P <0.01). There was no significant difference in the NFB cell cycle between AFB group and blank control group. CONCLUSION: WTI inhibits AFB proliferative activity by arresting AFB in G0 and G1 phases, delaying its entry into S phase, and WTI exerts stronger effects on AFB than NFB.