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目的研究磷酸三邻甲苯酯(tri-ortho-cresyl phosphate,TOCP)对母鸡神经组织线粒体膜通透性的影响,探讨有机磷诱导迟发性神经毒性的机制。方法成年罗曼母鸡随机分为4组,每组24只,TOCP溶解于玉米油中,染毒组分别按185、375、750mg/kg经灌胃一次性染毒,灌胃量为0.65ml/kg,对照组给予等体积玉米油。分别于染毒后第1、5、15、21天处死动物,迅速剥离母鸡大脑、小脑、脊髓组织,分离其线粒体,测定线粒体膜通透性和跨膜电位的改变。结果染毒组与对照组相比,大脑中线粒体膜通透性改变不明显;小脑375、750mg/kg组在染毒第1、5天时通透性有明显增加(P<0.05);染毒第1天时3个染毒组脊髓中线粒体膜通透性均明显增加(P<0.05),染毒第5、15天时375、750mg/kg组膜通透性明显增加(P<0.05)。各剂量组间及各时间点之间跨膜电位均未观察到明显改变。结论TOCP能明显增加母鸡小脑及脊髓中线粒体膜通透性。
Objective To investigate the effect of tri-ortho-cresyl phosphate (TOCP) on mitochondrial membrane permeability of hen’s nerve tissue and to explore the mechanism of organophosphate-induced delayed neurotoxicity. Methods Adult roman hen were randomly divided into 4 groups with 24 animals in each group. TOCP was dissolved in corn oil. The rats were exposed to 185,375 and 750 mg / kg, while the control group was given the same volume of corn oil. The animals were sacrificed on days 1, 5, 15, and 21 after exposure respectively. The hen brain, cerebellum and spinal cord tissues were rapidly dissected and their mitochondria were isolated. The mitochondrial membrane permeability and transmembrane potential were measured. Results Compared with the control group, the changes of mitochondrial membrane permeability in the brain were not obvious. The permeability of 375,750 mg / kg cerebrum increased obviously on the 1st and 5th day of exposure (P <0.05) The permeability of mitochondrial membrane in the spinal cord of the three treated groups was significantly increased on the first day (P <0.05). At the fifth and fifteenth day of exposure, the membrane permeability of 375 and 750 mg / kg group increased significantly (P <0.05). No significant changes were observed in transmembrane potential between each dose group and each time point. Conclusion TOCP can significantly increase mitochondrial permeability in the cerebellum and spinal cord of hen.