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目的研究血小板源性生长因子D(PDGF-D,-858、3166)位点基因多态性与尘肺发病的关系。方法选择确诊的96例Ⅰ期尘肺病患者为病例组,以接触同性质粉尘,工龄相近的非尘肺的接尘工人90例为对照组。采集周围静脉血,应用聚合酶链-限制性片段长度多态性(PCR-RFLP)技术检测PDGF-D(-858、3166)基因多态性。结果PDGF-D(-858)基因型(AA、AC、CC)在尘肺组的分布频率分别为63.54%、17.71%、18.75%,对照组分别为57.78%、16.67%、25.56%。PDGF-D(3166)基因型(CC、CG、GG)在尘肺组分布频率分别为54.17%、23.96%、21.88%,对照组分别为63.33%、12.22%、24.44%。分析发现PDGF-D(-858)基因型分布频率在尘肺组和对照组差异无显著性(P>0.05),PDGF-D(3166)基因型在尘肺组和对照组差异有显著性(P<0.05)。结论PDGF-D(3166)基因多态性可能与尘肺发病有关联。
Objective To investigate the relationship between the polymorphism of platelet-derived growth factor D (PDGF-D, -858,3166) and the incidence of pneumoconiosis. Methods Ninety - six patients with stage Ⅰ pneumoconiosis were selected as the case group. Ninety cases of non - pneumoconiosis workers who were exposed to the same type of dust and the same length of service were selected as the control group. Peripheral blood was collected and the polymorphism of PDGF-D (-858,3166) was detected by polymerase chain-restriction fragment length polymorphism (PCR-RFLP). Results The frequencies of PDGF-D (-858) genotypes (AA, AC, CC) in the pneumoconiosis group were 63.54%, 17.71% and 18.75% respectively, while those in the control group were 57.78%, 16.67% and 25.56%, respectively. The distribution frequencies of PDGF-D (3166) genotypes (CC, CG, GG) in pneumoconiosis group were 54.17%, 23.96% and 21.88% respectively, while those in control group were 63.33%, 12.22% and 24.44% respectively. The frequency of genotype distribution of PDGF-D (-858) was not significantly different between the pneumoconiosis group and the control group (P> 0.05). The genotype of PDGF-D (3166) was significantly different between the pneumoconiosis group and the control group (P < 0.05). Conclusion The polymorphism of PDGF-D (3166) may be associated with the pathogenesis of pneumoconiosis.