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目的:探讨正常妊娠与自然流产小鼠体内Th17/Treg细胞的表达差异以及白细胞介素-17(IL-17)对正常妊娠小鼠胚胎丢失的影响。方法:孕4 d时分别给予正常妊娠小鼠(CBA/J♀×BALB/c♂)生理盐水和IL-17A构建正常妊娠组和给药组,给予自然流产小鼠(CBA/J♀×DBA/2♂)生理盐水构建自然流产组。孕14 d时计算胚胎吸收率,采用ELISA法测定外周血IL-17水平,Real-time PCR及Western blot法检测胎盘组织IL-17、RORγt及Foxp3的表达水平。结果:自然流产组及给药组胚胎吸收率、胎盘组织中IL-17、RORγt的mRNA及蛋白水平显著高于正常妊娠组,而Foxp3水平则低于正常妊娠组;自然流产组外周血IL-17含量显著高于正常妊娠组。结论:自然流产小鼠体内Th17/Treg平衡明显向Th17细胞偏移,IL-17是介导流产发生的独立危险因素之一。
Objective: To investigate the difference of Th17 / Treg cells in normal pregnancy and spontaneous abortion mice and the effect of interleukin-17 (IL-17) on the embryo loss in normal pregnancy mice. Methods: Normal pregnant and control groups were given normal pregnant mice (CBA / J♀ × BALB / c♂) and IL-17A at 4 d after pregnancy, and spontaneous abortion mice (CBA / J♀ × DBA / 2♂) saline to construct a spontaneous abortion group. The embryo absorptance was calculated on the 14th day of gestation. The level of IL-17 in peripheral blood was measured by ELISA. The expression of IL-17, RORγt and Foxp3 in placenta were detected by Real-time PCR and Western blot. Results: Absorption rate of embryos in spontaneous abortion group and administration group were significantly higher than those in normal pregnancy group, but the levels of Foxp3 mRNA and protein in placental tissue were significantly lower than those in normal pregnancy group. IL- 17 content was significantly higher than normal pregnancy group. Conclusion: The balance of Th17 / Treg in spontaneous abortion mice obviously shifts to Th17 cells. IL-17 is one of the independent risk factors of abortion.