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目的探讨核因子-κB(NF-κB)在慢性髓细胞白血病(CML)急变期前后的表达及意义。方法选择2011年2月~2013年2月于新疆喀什人民医院血液科住院治疗的25例慢性髓细胞白血病慢性期(CML-CP)(CML-CP组)、16例慢性髓细胞白血病急变期(CML-BC)患者(CML-BC组)、30例缺铁性贫血或巨幼细胞性贫血患者(对照组)为研究对象。采用凝胶迁移或电泳迁移率实验检测三组人骨髓细胞中NF-κB的表达。结果对照组患者的NF-κB活性测定均为阴性,CML-CP组也仅4例患者检测到NF-κB活性增强,CML-BC组14例患者的NF-κB活性增强。CML-BC组患者NF-κB活化率明显高于CML-CP组及对照组,差异均有统计学意义(P<0.05或P<0.01);CML-CP组活化率高于对照组,差异有高度统计学意义(P<0.01)。CML-BC组患者NF-κB m RNA表达水平(22.91±0.15)明显高于对照组(0)及CML-CP组(11.19±0.03),差异有统计学意义(P<0.05)。结论与慢性髓细胞白血病慢性期比较,急变期NF-κB活性增强,表达增加,可作为慢性髓细胞白血病急性病变的重要标志物。
Objective To investigate the expression of nuclear factor-κB (NF-κB) in chronic myeloid leukemia (CML) before and after the blast crisis and its significance. Methods Twenty-five chronic myeloid leukemia (CML-CP) patients (CML-CP group) and 16 chronic myeloid leukemia patients were treated in Department of Hematology, Kashi People’s Hospital of Xinjiang from February 2011 to February 2013. CML-BC patients (CML-BC group) and 30 patients with iron-deficiency anemia or megaloblastic anemia (control group). Gel migration or electrophoretic mobility shift assay was used to detect NF-κB expression in three groups of human bone marrow cells. Results The NF-κB activity was negative in the control group. NF-κB activity was also detected in only 4 patients in CML-CP group, while NF-κB activity was enhanced in 14 patients in CML-BC group. The activation rate of NF-κB in CML-BC group was significantly higher than that in CML-CP group and control group (P <0.05 or P <0.01), and the activation rate in CML-CP group was higher than that in control group Highly statistically significant (P <0.01). The expression of NF-κB m RNA in CML-BC group was significantly higher than that in control group (0) and CML-CP group (11.19 ± 0.03) (22.91 ± 0.15), the difference was statistically significant (P <0.05). Conclusions Compared with the chronic myeloid leukemia chronic phase, the activity of NF-κB is increased and expressed in the blastic phase, which may be used as an important marker of acute myeloid leukemia.