论文部分内容阅读
目的探讨不同肝纤维化(LF)分级患者外周血单个核细胞Bcl-2蛋白表达及其临床意义。方法47例LF患者(男17例,女30例)与35例(男24例,女11例)同期住院治疗的慢性乙型肝炎无LF及41例(男29例,女12例)正常对照进行比较研究。以流式细胞仪双标记免疫荧光方法测定外周血T、B淋巴细胞Bcl-2蛋白表达和CD3+T细胞Bcl-2蛋白表达的平均荧光强度(MFI)。结果1.LF组CD3+、CD4+、CD8+T淋巴细胞亚群和CD19+细胞Bcl-2蛋白表达明显高于慢性乙型肝炎无肝纤维化组和对照组,差异有统计学意义(P<0.01)。外周血CD3+T细胞蛋白表达Bcl-2的MFI为0.61±0.17亦明显高于慢性乙型肝炎无肝纤维化组0.32±0.11和对照组的0.19±0.15(P<0.01)。2.Spearman相关分析显示CD3+、CD4+、CD8+T淋巴细胞亚群及CD19+细胞的Bcl-2蛋白表达与肝纤维化患者年龄、病程无明显相关,而与肝纤维化分期密切相关。3.抗病毒治疗连续1年,LF患者临床症状改善,且其淋巴细胞亚群Bcl-2蛋白表达亦较治疗前有明显下降(P<0.01),CD3+T细胞蛋白表达的MFI从0.61±0.17减少到0.43±0.14(P<0.01)。结论外周血淋巴细胞Bcl-2蛋白异常表达对LF发病及临床症状有重要作用。
Objective To investigate the expression of Bcl-2 protein in peripheral blood mononuclear cells in patients with different liver fibrosis (LF) grading and its clinical significance. Methods Forty-seven patients with LF (17 males and 30 females) and 35 normal controls (24 males and 11 females) were enrolled in this study. There were no chronic hepatitis B and 41 normal controls (29 males and 12 females) Make a comparative study. The average fluorescence intensity (MFI) of Bcl-2 protein expression in peripheral blood T, B lymphocytes and Bcl-2 protein expression in CD3 + T cells were determined by flow cytometry double-labeled immunofluorescence assay. Results 1. The expression of Bcl-2 protein in CD3 +, CD4 +, CD8 + T lymphocyte subsets and CD19 + cells in HL group were significantly higher than those in non-hepatic fibrosis group and control group (P <0.01) . The MFI of peripheral blood CD3 + T cell protein Bcl-2 was 0.61 ± 0.17, which was also significantly higher than that of chronic hepatitis B non-hepatic fibrosis group (0.32 ± 0.11) and control group (0.19 ± 0.15) (P <0.01). Spearman correlation analysis showed that the expression of Bcl-2 in CD3 +, CD4 +, CD8 + T lymphocyte subsets and CD19 + cells was not related to the age and course of liver fibrosis, but closely related to the stage of liver fibrosis. Antiviral therapy for 1 consecutive year, LF patients with clinical symptoms improved, and the lymphocyte subsets Bcl-2 protein expression was significantly lower than before treatment (P <0.01), MFI of CD3 + T cell protein expression from 0.61 ± 0.17 to 0.43 ± 0.14 (P <0.01). Conclusion Abnormal expression of Bcl-2 protein in peripheral blood lymphocytes plays an important role in the pathogenesis of LF and clinical symptoms.