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目的 :探讨急性淋巴细胞白血病 (ALL)的复发与P16蛋白缺失和bcl 2蛋白及血清可溶性白细胞介素 2受体 (sIL 2R)表达的关系。方法 :采用双抗体夹心酶联免疫吸附法 (ELISA)测定sIL 2R ,采用免疫组织化学方法测定P16蛋白、bcl 2蛋白的表达水平。结果 :在ALL中 ,P16蛋白缺失率、bcl 2蛋白阳性率及sIL 2R表达 ,复发组显著高于初诊组 (P <0 .0 1) ,初诊组显著高于完全缓解组 (CR组 ) (P <0 .0 1) ;初诊和复发ALL患者P16蛋白缺失率、bcl 2蛋白及sIL 2R表达与外周血白细胞总数 (r =0 .779,0 .886 ,0 .86 0 )、外周血幼稚细胞比值 (r =0 .82 1,0 .80 9,0 .813)和肝脾肿大呈正相关 (r =0 .84 6 ,0 .75 5 ,0 .84 0 ) (P <0 .0 1)。结论 :监测P16蛋白缺失、bcl 2蛋白及sIL 2R表达水平可预测ALL的复发。
Objective: To investigate the relationship between the relapse of acute lymphoblastic leukemia (ALL) and the loss of P16 protein and the expression of bcl 2 protein and serum soluble interleukin 2 receptor (sIL 2R). Methods: sIL 2R was detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The expression of P16 protein and bcl 2 protein were detected by immunohistochemistry. Results: The deletion rate of P16, the positive rate of bcl 2 protein and the expression of sIL 2R in ALL group were significantly higher than those in the newly diagnosed group (P <0.01), but significantly higher in the newly diagnosed group than those in the complete remission group (CR group) P <0.01). The rates of P16 protein deletion, the expression of bcl-2 protein and sIL 2R in peripheral blood and the total number of peripheral white blood cells (r = 0.779, 0.886, 0.86 0) The cell ratio (r = 0.82 1,0 .80 9,0. 813) was positively correlated with hepatosplenomegaly (r = 0.84 6, 0.755, 0.84 0) (P <0. 0 1). Conclusion: It is possible to predict the relapse of ALL by monitoring the expression of P16 protein, bcl-2 protein and sIL 2R.