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[Object] To investigate the optimum extraction conditions of polysaccharide from Eucommia ulmoides Oliver Leaves by ultrasonic and its anti-fatigue effect on Bufo gargarizans.[Method] The orthogonal test was designed to screen the optimum extraction process;the anti-fatigue effects of polysaccharides with different concentrations on toad were investigated by electric stimulation method.[Result] The optimum extraction conditions of polysaccharide were as follows:extracting temperature of 80 ℃,extracting time of 40 min,material-liquid ratio of 1:35,extracting times of 2.Compared with control,the maximum contraction time of toad muscle was shorten distinctly(P<0.05)and the muscular fatigue was alleviated(P<0.05;P<0.01)in polysaccharide group.[Conclusions] The muscle functions of toad could be improved by the polysaccharide from Eucommia ulmoides Oliver Leaves.
[Object] To investigate the optimum extraction conditions of polysaccharide from Eucommia ulmoides Oliver Leaves by ultrasonic and its anti-fatigue effect on Bufo gargarizans. [Method] The orthogonal test was designed to screen the optimum extraction process; the anti-fatigue effects of polysaccharides with different concentrations on toad were investigated by electric stimulation method. [Results] The optimum extraction conditions of polysaccharide were as follows: extraction temperature of 80 ° C, extraction time of 40 min, material-liquid ratio of 1: 35, extraction times of 2 .Compared with control, the maximum contraction time of toad muscle was shorten distinctly (P <0.05) and the muscular fatigue was alleviated (P <0.05; P <0.01) in polysaccharide group. [Conclusions] The muscle functions of toad could be improved by the polysaccharide from Eucommia ulmoides Oliver Leaves.