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目的建立更接近临床的模拟人工关节感染的动物模型。方法以健康成年ＳＤ大鼠为研究对象，在膝关节内置入的假体表面种植不同剂量的表皮葡萄球菌，通过细菌培养以确定能导致假体感染的稳定细菌剂量。结果在膝关节内置入假体后，发现致感染的细菌剂量明显小于无假体者，而且置入假体后，能产生细胞外多粘质物质（ｅｘｔｒａｃｅｌｕｌａｒｓｌｉｍｅｓｕｂｓｔａｎｃｅ，ＥＳＳ）的表皮葡萄球菌ＳＬ－７６亚型致感染的作用，明显强于不能产生ＥＳＳ的表皮葡萄球菌Ｇ２亚型。结论在金属假体置入关节后，选用１×１０４ｃｆｕ表皮葡萄球菌ＳＬ－７６就可建立假体感染的动物模型，其重复性强，所取得的标本可用于实验研究。
Objective To establish a more animal model of simulated artificial joint infection. Methods Healthy adult Sprague-Dawley rats were used as research objects. Different doses of Staphylococcus epidermidis were planted on the surface of the prosthesis inserted in the knee. Bacterial culture was used to determine the stable bacterial dose that could lead to prosthesis infection. Results After the prosthesis was inserted into the knee joint, the dose of bacteria causing the infection was found to be significantly lower than that of the non-prosthesis, and after implantation of the prosthesis, Staphylococcus epidermidis SL-76 producing extracelular lipids (ESS) Subtype-induced infection is significantly stronger than the Staphylococcus epidermidis G2 subtype that does not produce ESS. Conclusion The animal model of prosthesis infection can be established by using 1 × 104 cfu Staphylococcus epidermidis SL-76 after the metal prosthesis is inserted into the joint. The repeatability is strong. The obtained specimens can be used for experimental study.