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地衣素(Orcein)染色显示石蜡切片内的乙型肝炎表面抗原方法:石蜡切片厚4～5微米,常规用二甲苯脱蜡到水,用0.25％高锰酸钾硫酸液氧化5分钟(0.5％高锰酸钾水溶液,0.5％硫酸水溶液,两液在临用时等分混合,用滴管滴入盖过切片,每一玻片约需0.5～1毫升巳足),入水稍洗,以2％草酸漂白5分钟,流水稍冲洗2～3分钟,70％酒精稍洗,置入酸化的地衣素酒精液内染3～5小时(地衣素1克,70％酒精100毫升,浓盐酸0.84毫升,先把浓盐酸倾入70％酒精,然后加入地衣素,搅拌溶解,翌日即可使用),70％酒精漂洗二次,每次约半分钟,常规脱水透明,最后以中性树胶封固。结果:肝细胞内的地衣素阳性物质染为深棕色或紫棕色,呈微细颗粒状或均质状。阳性物质可形成圆形或椭圆形的团块,也可充满整个胞浆,呈环形或半
Orcein Staining for Hepatitis B Surface Antigen Displayed in Paraffin Ways Method: Paraffin sections are 4 to 5 microns thick and dewaxed conventionally with xylene to water, oxidized with 0.25% potassium permanganate sulfuric acid for 5 minutes (0.5% Aqueous solution of potassium permanganate, 0.5% aqueous sulfuric acid solution, the two liquid in the temporary use of equal mixture, drip covered with a dropper section, each slide about 0.5 to 1 ml of foot) enough water into the wash slightly to 2% Oxalic acid bleach for 5 minutes, water rinse for 2 to 3 minutes, 70% alcohol wash slightly, placed acidified lichen prime alcohol liquid dye 3 to 5 hours (lichen prime 1 g, 70% ethanol 100 ml, concentrated hydrochloric acid 0.84 ml, First concentrated hydrochloric acid into 70% alcohol, then add licorice, stirring to dissolve the next day you can use), 70% alcohol rinse twice, each about half a minute, conventional dewatering transparent, and finally sealed with a neutral gum. Results: The lichen-like positive substance in liver cells was stained dark brown or purple-brown, showing fine granular or homogeneous shape. Positive material can form round or oval clumps, but also can fill the entire cytoplasm, was ring or half