论文部分内容阅读
一、用新鲜猪脾先制成丙酮粉,继以重蒸水提取,经超速离心、超过滤及葡聚糖凝胶G-25凝胶柱层析,可分离得纯度较高的淋巴细胞抑素,其分子量大约是1400,活性物质中含有肽及核苷酸成分。 二、在离体细胞培养的条件下,培养基中的血清来源和在培养基中加入不同剂量的胰岛素,对淋巴细胞抑素的作用均无影响。 三、淋巴细胞抑素对淋巴细胞受PHA作用27小时的RNA合成有明显抑制作用,提示淋巴细胞抑素抑制淋巴细胞增殖的机制可能是抑制某些基因的激活过程,从而抑制DNA复制,使细胞不能从G_1期向S期过渡。
First, with fresh pigs spleen made acetone powder, followed by heavy distilled water extraction, ultracentrifugation, ultrafiltration and Sephadex G-25 gel column chromatography can be isolated from the higher purity of lymphocytes Elemental, its molecular weight is about 1400, the active substance contains peptides and nucleotide components. Second, under the conditions of ex vivo cell culture, the serum sources in the culture medium and the addition of different doses of insulin in the culture medium have no effect on the effect of lymphokistatin. Lymphocytosin on lymphocytes by PHA 27 hours of RNA synthesis was significantly inhibited, suggesting that lymphocytokine inhibition of lymphocyte proliferation may be the mechanism of inhibition of the activation of certain genes, thereby inhibiting DNA replication, so that cells Can not transition from G_1 phase to S phase.