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为探讨硫代乙酰胺(TAA)诱导草鱼肝胰脏损伤与损伤修复的作用机制,试验以腹腔注射硫代乙酰胺诱导肝胰脏损伤实验模型草鱼为对象,分别饲喂含有酵母培养物dv、姜黄素和水飞蓟素的饲料70 d后,采集草鱼肝胰脏样品,采用实时定量反转录聚合酶链式反应(qPCR)方法,检测了草鱼肝胰脏脂肪酸合成酶(FAS)、过氧化物酶增殖体激活受体γ辅助激活因子(PGC1-α)、硬脂酰辅酶A去饱和酶1(SCD1)、解偶联蛋白2(UCP2)、过氧化物酶增殖体激活受体α(PPAR-α)、过氧化物酶增殖体激活受体γ(PPAR-γ)、类胰岛素样生长因子(IGF-Ⅰ)的mRNA丰度,探讨在饲料中添加酵母培养物dv、姜黄素和水飞蓟素后,草鱼肝胰脏脂代谢相关基因表达丰度的变化。结果显示,TAA诱导草鱼肝损伤后,FAS基因表达丰度上调,调控脂质分解代谢的上游基因(如PPAR-α、ΡΡΑR-γ、PGC1-α)表达丰度没有显著性的变化,而SCD1基因表达丰度显著性下调,其结果可能导致脂质在肝细胞积累量增加,诱发脂肪性肝病的发生和发展。在饲料中添加酵母培养物dv、姜黄素和水飞蓟素后,在一定程度上修复了TAA对肝胰脏脂代谢的损伤。
To investigate the mechanism of thioacetamide (TAA) -induced liver-pancreas injury and injury repair in grass carp, the experiment was conducted by intraperitoneal injection of thioacetamide-induced hepatopancreas injury experimental model grass carp, respectively, fed with yeast culture dv, Curcumin and silymarin were collected for 70 days. The samples of liver and pancreas of grass carp were collected and analyzed by real-time quantitative reverse transcription-polymerase chain reaction (qPCR). The activities of fatty acid synthase (FAS), peroxidase (PGC1-α), SCD1, UCP2, PPAR- α, PPAR-γand IGF-Ⅰ mRNA abundance in the diet were investigated. After adding yeast culture dv, curcumin and silymarin to the feedstuff, Changes in abundance of lipid metabolism related genes in hepatopancreas of grass carp. The results showed that the expression of FAS gene was up-regulated in TAA-induced liver injury and no significant change was observed in the expression of upstream genes regulating lipid metabolism (such as PPAR-α, ΡΡΑR-γ, PGC1-α) Significantly decreased gene expression abundance, the results may lead to lipid accumulation in the liver cells increased, the incidence of fatty liver disease and development. The addition of yeast culture dv, curcumin and silymarin to the feed, to some extent, restored the damage of TAA to the hepatic and pancreatic lipid metabolism.