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为比较由抗-Ⅱ型胶原抗体诱导和抗耳蜗抗体被动转移所产生的耳蜗改变,作者选用体重300～500g 的健康豚鼠30只进行实验。第一组(10只)为抗耳蜗组,肌注抗豚鼠耳蜗的免疫球蛋白;第二组(10只)系抗-Ⅱ型胶原组,肌注纯化鸡Ⅱ型胶原和完全弗氏佐剂的混悬液;第三组(10只)为对照组,在同样实验条件下不加任何免疫刺激。另用体重1.5Kg 的兔子3只致敏及获取抗豚鼠耳蜗的抗体,即向兔子注入豚鼠膜性耳蜗浸出液与弗氏佐剂混悬液,使之免疫化后,分离出血清中的免疫球蛋白。耳蜗功能藉脑干反应测听作评价,组织损
To compare the cochlear changes induced by anti-type II collagen antibodies and passive transfer of anti-cochlear antibodies, we selected 30 healthy guinea pigs weighing 300-500 g for experiments. The first group (10 mice) was anti-cochlear group, intramuscular injection of anti-guinea pig cochlear immunoglobulin; the second group (10 mice) anti-type II collagen group, intramuscularly purified chicken type Ⅱ collagen and complete Freund’s adjuvant The third group (10 rats) was the control group, without any immunostimulation under the same experimental conditions. Another 1.5Kg rabbit body weight three sensitization and access to anti-guinea pig cochlear antibody, that is, to the rabbit guinea pig membranous cochlear extract leachate and Freund’s adjuvant suspension to immunization, the separation of serum immunoglobulin protein. Cochlear function by brainstem response audiometry for evaluation, tissue damage