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为确定不伴或伴肝外显性临床疾病的丙型肝炎病毒(HCV)感染者的血循环是否出现免疫复合物(IC),作者测定了健康献血者血液溶血活性,并评价了溶血活性的下降与HCV、HBV及其他献血常规筛选的病毒感染的血清学标志的关系。 10532份健康献血者血清,检测其HCV、HBV、HIV及HTLV-Ⅰ(Ⅰ型人T细胞白血病病毒)抗体及ALT。血清置4℃过夜后用微量滴定法测溶血活性;抗-HCV用被动血凝法测定;HCV RNA用巢式
To determine if immune complexes (ICs) occur in the bloodstream of patients with hepatitis C virus (HCV) infection in patients with and without overt or clinically apparent liver disease, the authors measured hemolytic activity in healthy blood donors and evaluated the decrease in hemolytic activity And HCV, HBV and other blood donation routine screening of serological markers of infection. The serum of 10532 healthy donors was tested for their antibodies to HCV, HBV, HIV and HTLV-Ⅰ (type Ⅰ human leukemia virus) and ALT. Serum was incubated overnight at 4 ℃ with a microtiter method for the determination of hemolysis activity; anti-HCV was determined by passive hemagglutination; HCV RNA was nested