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目的建立妊娠及哺乳期铅摄入对后代学习记忆损害的动物模型的影响。方法母鼠在妊娠及哺乳期的不同阶段给予铅暴露后观察子鼠的学习记忆能力的变化,孕鼠70只,自确定受孕后即随机分成7组。孕早期低剂量组(60mg/kg)和孕早期高剂量组(240mg/kg),于受孕第0天给醋酸铅直至仔鼠断乳,其染铅42d;孕晚期低剂量组(60mg/kg)和孕晚期高剂量组(240mg/kg),于受孕第14天起给醋酸铅至仔鼠断乳,共染铅28d;出生后低剂量组(60mg/kg)和高剂量组(240mg/kg),于分娩日起给醋酸铅至仔鼠断乳,共染铅21d;另设阴性对照组。结果大鼠在孕期和哺乳期给铅致20日龄后代幼鼠的定位航行能力受到明显影响。随着训练时间的增加,对照组的幼鼠逃避时间显著缩短,而在孕期和哺乳期给铅致20日龄各组后代幼鼠的逃避时间并没有显著缩短,与对照组比较,差异具有统计学意义。在训练7d后进行空间探索测试,对照组幼鼠搜寻平台的时间明显较实验各组快,差异具有统计学意义;穿越平台的次数对照组也显著多于实验各组,差异有统计学意义;在第Ⅰ象限停留的时间比实验各组要长,差异有统计学意义。结论母鼠妊娠及哺乳期铅处理的各组幼鼠空间学习记忆能力有明显的影响,此模型适合进一步研究围产期铅暴露后子代学习记忆的影响。
Objective To establish the effect of lead intake during pregnancy and lactation on the animal model of learning and memory impairment in the offspring. Methods The pregnant rats were exposed to lead at different stages of pregnancy and lactation to observe the changes of learning and memory ability of the offspring. 70 pregnant rats were randomly divided into 7 groups after the conception. In the early pregnancy low dose group (60mg / kg) and early pregnancy high dose group (240mg / kg), on the 0th day of pregnancy, lead acetate was weaned until the pups were weaned, (240mg / kg) and the lead acetate to the offspring were weaned on the 14th day after pregnancy, and the total lead exposure time was 28 days. After birth, the low dose group (60mg / kg) and high dose group (240mg / kg) , From the date of delivery to give off lead acetate to offspring, a total of 21d lead; another negative control group. Results The positioning and navigation ability of the 20-day-old offspring rats during pregnancy and lactation were significantly affected. With the increase of training time, the escape time of rats in the control group was significantly shortened, but the escape time of the offspring of the 20-day-old lead generation was not significantly shortened during pregnancy and lactation, the difference was statistically significant compared with the control group Significance of learning. The space exploration test was performed 7 days after training. The search time of the control group was significantly faster than that of the experimental groups, the difference was statistically significant. The number of passing through the platform was also significantly more than that of the experimental groups, with significant difference; The time spent in the I quadrant was longer than that in the experimental group, with significant difference. Conclusion The spatial learning and memory abilities of pups in pregnancy and lactation induced by lead exposure in maternal rats are significantly affected. This model is suitable for further studying the influence of learning and memory of offspring after perinatal lead exposure.