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目的 探讨如何将表位构建成有效免疫原。方法 以Pre S( 2 ) 蛋白两优势性B细胞表位和HBcAg两Th细胞表位为基础 ,设计、合成了 2种MAP多肽 ,以此两MAP肽为免疫原免疫兔和小鼠 ,观察体液免疫反应。结果 此两种MAP多肽均比Pre S( 2 ) 蛋白诱发更高抗体滴度 ;Th细胞表位引入可显著增强反应强度。结论 增加肽抗原结构复杂性可提高其免疫原性 ,其表位排列以B细胞表位在赖氨酸核心外侧为佳 ,MAP是一种较好的肽苗结构
Objective To explore how to construct epitopes into effective immunogens. Methods Two kinds of MAP polypeptides were designed and synthesized based on two predominant B cell epitopes of Pre S (2) protein and HBcAg two Th cell epitopes. Two MAP peptides were used as immunogen to immunize rabbits and mice, and body fluid immune response. Results Both MAP polypeptides induced higher antibody titers than Pre S (2) protein; the introduction of Th-cell epitopes significantly enhanced the reaction intensity. Conclusions Increasing the peptide antigen structure complexity may enhance its immunogenicity. The epitopes are arranged on the outer side of the lysine core with B cell epitopes, and MAP is a good peptide vaccine structure