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目的研究江西省2013年麻疹病毒的基因型别和特征,掌握江西省麻疹病毒的基因型别和流行特点。方法采用Vero/SLAM细胞分离培养麻疹病毒,用RT-PCR方法扩增麻疹病毒N基因,并进行序列测定和同源性分析。结果2013年江西省分离的15株麻疹病毒株均为H1a基因亚型,与H1基因的同源性为97.3%~98.1%;与中国疫苗株Shanghai-191株的N基因核苷酸同源性为93.7%~94.7%。结论 H1a基因亚型为江西省本土流行病毒株的优势亚型,近年来江西省流行的麻疹病毒无较大差异。
Objective To study the genotypes and characteristics of measles virus in Jiangxi Province in 2013 and to know the genotype and epidemic characteristics of measles virus in Jiangxi Province. Methods The measles virus was isolated and cultured in Vero / SLAM cells. The gene of measles virus N was amplified by RT-PCR and sequenced and analyzed for homology. Results The 15 isolates of measles isolates from Jiangxi province in 2013 were all H1a subtypes with a homology of 97.3% ~ 98.1% with H1 gene. The homology of N gene with that of Chinese strain Shanghai-191 93.7% ~ 94.7%. Conclusion H1a subtype is the predominant subtype of local epidemic strain in Jiangxi Province. There is no significant difference in the prevalence of measles virus in Jiangxi Province in recent years.