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海南省１９８５－１９９３年经历了４次较大的脊髓灰质炎（脊灰）流行，年发病数分别为７２５例（１９８５年）１９７例（１９８９年；６４例（１９９０年）和６５例（１９９３）年．历年流行的野毒株均为脊灰Ｉ型病毒．对历年来分离的病毒株先用ＰＣＲ－ＲＦＬＰ法选出有代表性的毒株，再将这些病毒株ＶＰ１基因的部份片段做核酸序列分析，并与非流物年分离的毒株作相应的比较。结果显示，每次较大流行年的分离毒株与上一次流行的代表株相比较，其核苷酸均有效大的改变，说明当脊灰新的基因型出现时便在本省有一次较大的流行，对新的基因型脊灰病毒的由来，结合全国脊灰病毒的分子流行病学资料进行了讨论。
Four major poliomyelitis (polio) epidemics were reported in Hainan Province from 1985 to 1993, with an annual incidence of 725 cases (1985), 197 cases (1989; 64 cases (1990) and 65 cases ) Years.The prevalence of wild strains over the years are poliovirus type I. To isolate strains over the years by PCR-RFLP first selected representative strains, and then these strains of VP1 gene fragments The results of nucleic acid sequence analysis showed that the nucleotide sequences of the isolated strains in each major pandemic year were more effective than those of the last popular representative strain , Indicating that there was a major epidemic in the province when the new genotype of polio appeared and the origin of the new genotype of poliovirus was discussed in conjunction with the molecular epidemiological data of the national poliovirus.