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目的:探讨超抗原葡萄球菌肠毒素B(SEB)诱导的耐受性是否参与免疫赦免部位的应答反应。方法:以LEW大鼠作为受体,F344大鼠作为供体,进行角膜移植。将受体鼠随机分成5组,即在手术前7 d及移植后7 d,以球旁途径分别注射30μg/kg、60μg/kg、90μg/kg和120μg/kg的SEB组和注射生理盐水的阴性对照组。阳性对照组分别为移植后注射糖皮质激素(GC)、FK506、环孢素A((CsA)和白介素-1受体拮抗剂(IL-1 ra)组。镜下观察移植后30 d内角膜的浑浊、水肿和新生血管,用抗排斥反应指数记录移植片存活天数。采用NK1.1-PE荧光抗体进行组织染色,观察移植后不同时间受体鼠角膜片的炎症细胞浸润和受体鼠眼局部NK细胞的变化。结果:注射120μg/kg SEB的大鼠移植片比生理盐水对照组的存活天数延长了22 d。与FK506、CsA、IL-1ra和GC组相比也明显延长。120μg SEB/kg受体鼠的移植片排斥反应指数为3.42±2.18,明显低于对照组6.58±3.15(P<0.01)。其中水肿指数和新生血管指数明显降低。组织染色结果显示,SEB注射后NK细胞数量增加。结论:注射SEB能降低大鼠角膜移植的排斥反应,提示SEB诱导的耐受性参与了免疫赦免部位的应答反应。
Aims: To investigate whether the tolerance induced by superantigen staphylococcal enterotoxin B (SEB) is involved in the response of immune pardons. Methods: LEW rats were used as recipients and F344 rats as donors for corneal transplantation. The recipient mice were randomly divided into five groups: SEB group injected with 30μg / kg, 60μg / kg, 90μg / kg and 120μg / kg respectively Negative control group. The positive control group was injected with glucocorticoid (GC), FK506, cyclosporine A (CsA) and interleukin-1 receptor antagonist (IL-1 ra) Of the turbidity, edema and neovascularization, anti-rejection index was recorded survival days of the graft.Using NK1.1-PE fluorescent antibody staining, observed at different times after transplantation recipient corneal inflammatory cell infiltration and receptor rat eye Local NK cells were observed.Results: The number of days of survival of rats injected with 120μg / kg SEB was 22 days longer than that of saline control group, significantly prolonged compared with that of FK506, CsA, IL-1ra and GC groups.120μg SEB / kg receptor implanted graft rejection index was 3.42 ± 2.18, significantly lower than the control group 6.58 ± 3.15 (P <0.01), which edema index and neovascular index was significantly decreased.The results of tissue staining showed that SEB injection of NK cells Conclusions: Injection of SEB can reduce the rejection of rat corneal transplantation, suggesting that SEB-induced tolerance is involved in the response of immunopapillary sites.