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目的探讨非甾体类抗炎药氮-2,环己氧-4,硝基苯-甲基磺胺(NS398)的可能抗肿瘤机制。方法成瘤裸鼠分为实验组和对照组,实验组口咽管喂NS398,每天0.1 mg/g,分别于10、20、30 d后处死,保留肿瘤组织。抽取组织总RNA,检测RECK基因与基质金属蛋白酶2(MMP-2)的表达;抽取组织总蛋白,用Western blot检测RECK蛋白的表达。结果实验组肿瘤组织中RECK基因的表达量明显升高,而MMP-2的表达量明显降低,肿瘤体积缩小。其变化与用药时间有明显的相关性,。结论 NS398对于前列腺癌的发生及转移有明显的抑制作用。其作用机制可能与其诱导RECK基因的表达,抑制MMP-2的表达有关。
Objective To investigate the possible antitumor mechanism of non-steroidal anti-inflammatory drugs, such as nitrogen-2, cyclohexyloxy-4 and nitrobenzene-methyl sulfonamide (NS398). Methods Tumor-bearing nude mice were divided into experimental group and control group. Oral pharyngeal tube was fed with NS398 in the experimental group, 0.1 mg / g per day. The tumor tissues were sacrificed at 10, 20 and 30 days respectively. Tissue RNA was extracted and the expression of RECK gene and matrix metalloproteinase 2 (MMP-2) were detected. Tissue protein was extracted and the expression of RECK protein was detected by Western blot. Results The expression of RECK gene in the experimental group was significantly increased, but the expression of MMP-2 was significantly reduced and the tumor volume was reduced. The change has obvious correlation with medication time. Conclusion NS398 has a significant inhibitory effect on the occurrence and metastasis of prostate cancer. Its mechanism may be related to its induction of RECK gene expression, inhibition of MMP-2 expression.