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目的:建立HPLC-ELSD同时测定猴头菌丝固体培养物及胃乐宁片中麦角甾醇和β-谷甾醇含量方法。方法:采用Kromasil C_(18)色谱柱(4.6 mm×250 mm,5μm),以甲醇为流动相,流速1 m L·min~(-1),柱温35℃,检测器为ELSD,漂移管温度70℃,载气压力275.8 k Pa。结果:麦角甾醇和β-谷甾醇峰面积的对数与浓度的对数呈良好的线性关系(r>0.999 2),猴头菌丝固体培养物中麦角甾醇和β-谷甾醇平均回收率分别为92.6%和94.1%;胃乐宁片中麦角甾醇和β-谷甾醇平均加样回收率分别为103.0%和94.6%。10批猴头菌丝固体培养物中麦角甾醇和β-谷甾醇的含量分别为0.081~0.178和0.101~0.128 mg·g~(-1);10批胃乐宁片中麦角甾醇和β-谷甾醇的含量分别为0~0.252和0~0.380 mg·g~(-1)。结论:该方法可用于猴头菌丝固体培养物及胃乐宁片质量控制。
Objective: To establish a HPLC-ELSD method for the simultaneous determination of ergosterol and β-sitosterol in Hericium erinaceus solid culture and Weilening tablets. Methods: Kromasil C 18 column (4.6 mm × 250 mm, 5 μm) was used with methanol as the mobile phase at a flow rate of 1 mL · min -1. The column temperature was 35 ℃. The detector was ELSD, drift tube Temperature 70 ℃, carrier gas pressure 275.8 k Pa. RESULTS: The logarithm of the peak area of ergosterol and β-sitosterol showed a good linear relationship with the logarithm of the concentration (r> 0.999 2). The mean recoveries of ergosterol and β-sitosterol in the solidified Hericium erinaceus were Which was 92.6% and 94.1%, respectively. The mean recoveries of ergosterol and β-sitosterol in Weilening tablets were 103.0% and 94.6%, respectively. The contents of ergosterol and β-sitosterol in 10 batches of hericium mycelium were 0.081 ~ 0.178 and 0.101 ~ 0.128 mg · g ~ (-1), respectively. Ten batches of ergosterol and β- The contents of sterols were 0 ~ 0.252 and 0 ~ 0.380 mg · g ~ (-1), respectively. Conclusion: This method can be applied to the quality control of Hericium spp. Solid culture and Weleining tablet.