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目的介绍一种改进的豚鼠灌胃给药方法。方法选取60只普通级豚鼠,灌胃时使豚鼠后肢站立于支持物,体位垂直且不能前移,灌胃针头顺两侧嘴角轻轻插入豚鼠口腔内部,插入深度为1.5 cm~2 cm,开始先轻轻推入0.2~0.5 ml药液,当看见豚鼠有咀嚼和吞咽动作时再推入适量药液。依次类推,直至将药液全部灌入豚鼠胃内。结果按照上述方法在30 d内,60只豚鼠每天1次,均能顺利实现灌胃。经解剖观察无消化道损伤,无误入气道导致死亡,成功率达到100%。平均每只动物灌胃时间为(6.8±2.4)s,耗费时间较短。结论改良的豚鼠灌胃方法大大减少了人为因素导致的动物死亡,使经口给药途径的动物实验不需要因为技术问题而局限于大鼠、小鼠,为进一步的医药学研究奠定了基础。
Objective To introduce an improved guinea pig gavage method. Methods Sixty normal guinea pigs were selected. Guinea pig hindlimbs were standing on the supporter while gavage was in place. The position of the guinea pigs was vertical and could not advance. The gunshot mouth was gently inserted into the oral cavity of guinea pigs with the insertion depth of 1.5 cm ~ 2 cm First gently push into 0.2 ~ 0.5 ml liquid, when you see the guinea pig chewing and swallowing movement and then push the amount of liquid. And so on, until the liquid poured into the guinea pig stomach. Results According to the above method within 60 days, 60 guinea pigs once a day, can be successfully achieved gavage. Anatomical observation of non-gastrointestinal injury, no accident into the airways leading to death, the success rate of 100%. The average time for each animal gavage was (6.8 ± 2.4) s, taking less time. Conclusion The improved guinea pig gavage method can greatly reduce animal death caused by human factors. Therefore, the animal experiment of oral administration does not need to be confined to rats and mice for technical problems, which lays the foundation for further medical research.