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为研究碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对晶体上皮细胞促增殖作用的机理，采用原位杂交，用合成的寡核苷酸探针来检测人晶体上皮细胞内的ｂＦＧＦ高亲和力受体—成纤维细胞生长因子受体１（ｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒｒｅ－ｃｅｐｔｏｒ１，ＦＧＦＲ１）的ｍＲＮＡ基因，并用图像分析进行相对定量。结果：表明了人晶体上皮细胞表达ＦＧＦＲ１的ｍＲＮＡ基因。结论：人晶体上皮细胞存在ＦＧＦＲ１，当ｂＦＧＦ与其高亲和力受体结合后，对促进晶体上皮细胞的增殖以及白内障术后后囊混浊的形成具有重要作用。
In order to investigate the mechanism of basic fibroblast growth factor (bFGF) promoting proliferation of human epithelial cells, in situ hybridization was used to detect the high affinity of bFGF in human lens epithelial cells using synthetic oligonucleotide probes The mRNA gene of fibroblast growth factor receptor-1 (FGFR1) was quantified by image analysis. Results: The mRNA expression of FGFR1 in human lens epithelial cells was demonstrated. Conclusion: FGFR1 is present in human lens epithelial cells. When bFGF binds to its high affinity receptor, it plays an important role in promoting the proliferation of lens epithelial cells and the formation of posterior capsule opacification after cataract surgery.