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研究目的为查明手淫回收精子前,低电解质溶液注入逆行射精患者的膀胱内的安全性和有效性 在行子宫受精日到门诊前1h内饮水3杯,插入导尿管排空膀胱内的尿液。注入0.32 mol/L葡萄糖溶液30 ml或改良Ham’sF-10培养剂(含钠146 mmol/L,钾4 mmol/L,pH=7.5)加10％灭活的患者血清注入膀胱内。手淫后立即再次插入导尿管吸取精液的混合液进行培养。混合液在150× g离心15 min后立即用喂养管将0.5 ml离心后沉淀的混合液经子宫颈注入子宫内。小量沉淀
The purpose of the study To find out the safety and effectiveness of low electrolyte solution injection into the bladder of patients with retrograde ejaculation before masturbation recovery of spermatozoa. Three cups of water were taken within 1h before uterine fertilization day and inserted into a catheter to empty the urine in the bladder liquid. Into the bladder was injected with 30 ml of 0.32 mol / L glucose solution or modified Ham’s F-10 culture medium (containing 146 mmol / L sodium, 4 mmol / L potassium, pH = 7.5) plus 10% inactivated sera. Immediately after masturbation, the catheter is again inserted into the catheter to extract the mixture of semen for culture. After the mixture was centrifuged at 150 × g for 15 min, 0.5 ml of the pelleted mixture was injected into the uterus through the cervix with a feeding tube. A small amount of precipitation