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目的:探讨柚皮素对阿尔茨海默病(AD)模型大鼠学习记忆能力的影响。方法:将30只雄性SD大鼠随机分为假手术组、模型组、柚皮素组。侧脑室注射链脲佐菌素(ICV-STZ)(3 mg/kg,共2次)制备AD模型。柚皮素组行柚皮素灌胃,其余两组生理盐水灌胃,1次/d,连续3 w。以Morris水迷宫测试各组大鼠学习记忆能力;测试完成后取各组大鼠脑组织,用化学比色法测定其超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;免疫组织化学染色法观察其Aβ42和Aβ40的表达情况;Western blotting法定量检测其Tau蛋白的表达及磷酸化程度。结果:水迷宫结果显示,与假手术组比较,模型组平均逃避潜伏期显著延长(P<0.05),在原平台活动时间显著缩短(P<0.05);与模型组比较,柚皮素组平均逃避潜伏期显著缩短(P<0.05),在原平台活动时间显著延长(P<0.05);化学比色法结果显示,模型组较假手术组肠组织SOD活性显著降低(P<0.05);而柚皮素组较模型组肠组织SOD活性显著升高(P<0.05);与假手术组比较,模型组肠组织MDA含量显著升高(P<0.05);与模型组比较,柚皮素组MDA含量显著降低(P<0.05);免疫组化结果显示,模型组Aβ40、Aβ42阳性表达较对照组显著增多(P<0.05),柚皮素组较模型组阳性表达显著降低(P<0.05);Tau蛋白表达在各组间无差异,但模型组Tau蛋白磷酸化程度较对照组显著增强(P<0.05),柚皮素组较模型组显著减弱(P<0.05)。结论:柚皮素可通过氧化应激途径较好地改善阿尔茨海默病模型大鼠的学习记忆能力。
Objective: To investigate the effect of naringenin on the learning and memory abilities of Alzheimer’s disease (AD) model rats. Methods: Thirty male SD rats were randomly divided into sham operation group, model group and naringenin group. AD model was prepared by intracerebroventricular injection of streptozotocin (ICV-STZ) (3 mg / kg, 2 times). Naringenin group naringenin gavage, the remaining two groups of saline gavage, 1 / d, for 3 w. The learning and memory abilities of rats in each group were tested by Morris water maze. After the test, the brain tissue of each group was taken out and the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured by chemical colorimetry. The expression of Aβ42 and Aβ40 were observed by histochemical staining. The expression of Tau protein and the degree of phosphorylation were detected by Western blotting. Results: The water maze results showed that the mean escape latency of the model group was significantly longer than that of the sham operation group (P <0.05), and significantly shorter on the original platform (P <0.05). Compared with the model group, the mean escape latency (P <0.05), and the activity time in the original platform was significantly prolonged (P <0.05). The results of chemical colorimetry showed that the activity of SOD in the model group was significantly lower than that in the sham operation group (P <0.05) Compared with the sham operation group, the content of MDA in intestinal mucosa in model group was significantly increased (P <0.05), and the content of MDA in naringenin group was significantly lower than that in model group (P <0.05). The results of immunohistochemistry showed that the positive expression of Aβ40 and Aβ42 in model group was significantly higher than that in control group (P <0.05), the expression of Tau protein in naringenin group was significantly lower than that of model group (P <0.05) There was no difference among the groups, but the phosphorylation of Tau in the model group was significantly higher than that in the control group (P <0.05). The naringenin group was significantly weaker than the model group (P <0.05). Conclusion: Naringenin can improve the learning and memory ability of Alzheimer’s disease model rats through oxidative stress.