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(加高脂血清(HL)培养小牛主动脉平滑肌细胞(SMC)以加强SMC内脂质过氧化作用,并在培养液中添加不同浓度的亚硒酸钠。以VE作对照。HL组SMC内过氧化脂质(Lpo)含量显著高于对照组,加入硒和VE,SMC内Lpo均低于高脂组及对照组。60℃一次照射小白鼠,全血谷胱甘硒过氧化物酶(GSH—Px)活性显著低于对照组;服硒及VE的放射小白鼠全血GSGPx活性显著高于放射组接近对照水平。体内外实验表明硒具有提高GSH—Px活性抗过氧化脂质损伤的作用。
(High-lipid serum (HL) was used to culture calf aortic smooth muscle cells (SMC) to enhance lipid peroxidation in SMCs, and different concentrations of sodium selenite were added to the culture medium. VE was used as a control. HL group SMC The content of lipid peroxide (Lpo) was significantly higher than that of the control group. Compared with sebum and VE, the Lpo in SMC was lower than that in the high fat group and the control group. The mice were irradiated once at 60°C and the whole blood was glutathione and selenium peroxidase. (GSH-Px) activity was significantly lower than that of the control group, and the activity of GSGPx in the whole blood of selenium- and VE-irradiated white mice was significantly higher than that of the irradiated group.Experimental studies in vivo and in vitro showed that selenium has the function of increasing GSH-Px activity against lipid peroxidation. The role.