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目的:研究白细胞介素23(IL-23)对强直性脊柱炎(AS)患者的临床诊断意义及其受体表达情况。方法:选择2012年11月至2013年11月丹江口市妇幼保健院收治的AS患者40例为AS组,另外选择同期健康体检者40例作为健康对照组。测定两组受试者血清IL-23、红细胞沉降率(ESR)、C反应蛋白(CRP)和免疫球蛋白(IgA)水平;收集分离两组受试者外周血中单个核细胞(PBMCs)并行体外细胞培养,收集细胞,利用Western blotting技术检测IL-23 p19R蛋白表达变化。结果:AS患者血清中IL-23水平显著高于健康对照组[(630±69)ng·L~(-1)vs(84±7)ng·L~(-1),P<0.05],且AS组血清IL-23水平与Bath AS疾病活动指数(BASDAI)、ESR和CRP的浓度呈正相关(r值分别为0.598、0.602、0.548、0.436,P值分别为0.004、0.005、0.040、0.030)。AS组PBMCs经IL-23刺激后,其IL-23 p19R蛋白表达显著升高。结论:AS患者血清中IL-23水平与BASDAI、ESR、CRP及IgA的水平变化呈正相关,IL-23可能通过改变IL-23 p19R蛋白的变化来介导CD_4~+T向Th17分化,参与AS的发病。
Objective: To investigate the significance of interleukin-23 (IL-23) in the diagnosis of ankylosing spondylitis (AS) and its receptor expression. Methods: From November 2012 to November 2013, 40 AS patients in Danjiangkou MCH hospital were selected as AS group, and another 40 healthy subjects were selected as healthy control group. Serum levels of IL-23, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and immunoglobulin (IgA) were measured in two groups of subjects; peripheral blood mononuclear cells (PBMCs) In vitro cell culture, collection of cells, the use of Western blotting detection of IL-23 p19R protein expression changes. Results: Serum levels of IL-23 in AS patients were significantly higher than those in healthy controls [(630 ± 69) ng · L -1 vs (84 ± 7) ng · L -1, P <0.05] Serum IL-23 level in AS group was positively correlated with Bath AS disease activity index (BASDAI), ESR and CRP concentrations (r values were 0.598,0.602,0.548,0.436, P values were 0.004,0.005,0.040,0.030 respectively) . The expression of IL-23 p19R protein in PBMCs of AS group was significantly increased after IL-23 stimulation. CONCLUSIONS: The level of IL-23 in serum of AS patients is positively correlated with the level of BASDAI, ESR, CRP and IgA. IL-23 may mediate the differentiation of CD_4 + T to Th17 by changing the expression of IL-23 p19R protein. The incidence.