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目的 :建立尿中 10种常见的苯骈二氮杂类药物 (氯硝西泮、氟硝西泮、艾司唑仑、三唑仑、阿普唑仑、硝西泮、去甲地西泮、奥沙西泮、替马西泮、地西泮 )的柱切换高效液相色谱测定方法。方法 :尿样用磷酸盐缓冲液 1∶1稀释 ,离心后直接进样1 0mL进入预处理柱 ,用水作为预处理流动相 ,然后用分析流动相CH3OH H2 O( 60∶40 )将保留在预处理柱上的药物反冲进入分析柱进行分析。检测波长 (λ)为 2 0 0nm。结果 :氯硝西泮、氟硝西泮、艾司唑仑、三唑仑、阿普唑仑的检测限均为 2ng·mL-1(rSN=3 ) ,线性范围为 10～ 10 0 0ng·mL-1,r≥ 0 .9992 ;硝西泮、奥沙西泮、替马西泮、去甲地西泮、地西泮的检测限为 5ng·mL-1(rSN=3 ) ,线性范围为 2 0～ 10 0 0ng·mL-1,r≥ 0 9993。日内、日间RSD均 <10 % (n =5 )。结论 :本法准确、灵敏、快速、简便 ,可直接用于尿中苯骈二氮杂类药物的检测
Objective: To establish 10 common benzodiazepines in urine (clonazepam, flunitrazepam, estazolam, triazolam, alprazolam, nitrazepam, Pam, Oxazepam, Temazepam, Diazepam) by column-switching high performance liquid chromatography. Methods: The urine samples were diluted 1: 1 with phosphate buffer solution and directly injected into the pretreatment column after centrifugation. The water was used as the pretreatment mobile phase, and then the mobile phase CH3OH H2 O (60:40) The drug on the treatment column is backflushed into the analytical column for analysis. The detection wavelength (λ) is 200 nm. Results: The detection limits of clonazepam, flunitrazepam, estazolam, triazolam and alprazolam were both 2 ng · mL-1 (rSN = 3) and the linear range was 10 ~ 100 ng · mL-1, r≥0.9999. The detection limit of nitrazepam, oxazepam, temazepam, noradazepam and diazepam was 5 ng · mL-1 (rSN = 3) 0 ~ 10 0 ng · mL-1, r ≥ 0 9993. Day and day RSD were <10% (n = 5). Conclusion: This method is accurate, sensitive, rapid and simple and can be directly applied to the detection of benzodiazepines in urine