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目的:评价骨髓基质细胞移植对心肌梗死后心肌间质纤维化的影响。方法:30只日本大耳白兔,随机分为细胞移植组、药物治疗组、对照组,体外骨髓穿刺提取、分离、培养骨髓基质细胞,心肌梗死模型制作成功2周后沿梗死周边区域注射细胞悬液100μl(细胞移植组)或DMEM培养基100μl(对照组)或给予安体舒通20mg,bid,服用1个月(药物治疗组)。各组实验前、后4周经心脏超声检查,氯胺T氧化法测定血浆、组织羟脯胺酸含量,TTC染色评价梗死面积。结果:细胞移植组血浆、组织羟脯胺酸含量[(22.79±1.69)mg/L、(1.59±0.89)μg/mg]均低于对照组[(40.16±2.31)mg/L、(3.59±0.19)μg/mg]、药物治疗组[(34.24±1.98)mg/L、(2.67±0.81)μg/mg],均P<0.05。实验后4周时,细胞移植组心功能有所提高[LVEF(56.91±2.04)%],与对照组[(32.49±1.29)%]、药物治疗组[(53.22±2.13)%]比较,均P<0.05;梗死面积[(22.82±3.12)%]有所下降,与对照组[(29.73±2.11)%]、药物治疗组[(28.61±1.24)%]比较,均P<0.05。结论:细胞移植后可抑制心肌胶原合成、抑制心肌局部间质纤维化,是细胞移植心功能改善机制之一。
Objective: To evaluate the effect of bone marrow stromal cell transplantation on myocardial interstitial fibrosis after myocardial infarction. Methods: Thirty Japanese white rabbits were randomly divided into cell transplantation group, drug treatment group and control group. Bone marrow stromal cells were extracted from bone marrow and cultured in vitro. After 2 weeks of successful myocardial infarction model, the cell suspension was injected along the peripheral region of infarction 100μl (cell transplantation group) or DMEM medium 100μl (control group) or spironolactone 20mg, bid, take 1 month (drug treatment group). Each group before and after 4 weeks by echocardiography, chloramine T oxidation of plasma and tissue hydroxyproline content, TTC staining infarct size. Results: Compared with the control group [(40.16 ± 2.31) mg / L and (3.59 ±)] mg / L, the content of hydroxyproline in plasma and tissue of the cell transplantation group was significantly lower than that of the control group [(22.79 ± 1.69) mg / 0.19) μg / mg], and (34.24 ± 1.98) mg / L and (2.67 ± 0.81) μg / mg respectively in the drug treatment group, all P <0.05. Compared with control group [(32.49 ± 1.29)%] and drug treatment group [(53.22 ± 2.13)%], the cardiac function of the cell transplantation group was improved at 4 weeks after the experiment [LVEF (56.91 ± 2.04)%] P <0.05; infarction area [(22.82 ± 3.12)%] was decreased, P <0.05 compared with the control group [(29.73 ± 2.11)%] and the drug treatment group (28.61 ± 1.24)%. CONCLUSION: Cell transplantation can inhibit collagen synthesis and inhibit myocardial fibrosis, which is one of the mechanisms of cardiac function improvement.