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目的：探讨ＷＴ１基因在白血病细胞中的表达及其临床意义。方法：应用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）法测定Ｋ５６２、ＨＬ－６０、ＴＦ－１及Ｕ９３７四株白血病细胞系，４５例白血病患者，１５名正常人外周血及１０例非血液病患者正常骨髓细胞ＷＴ１基因的表达。结果：Ｋ５６２、ＨＬ－６０及ＴＦ－１三株白血病细胞系均高度表达ＷＴ１，而Ｕ９３７细胞系未测到ＷＴ１。急性髓系白血病（ＡＭＬ）２５例中２１例、急性淋巴细胞白血病（ＡＬＬ）１１例中８例、慢性粒细胞白血病（ＣＭＬ）急性变者２例、慢性及加速期ＣＭＬ患者７例中１例ＷＴ１表达阳性。１５名正常人外周血及１０例非血液病患者正常骨髓细胞ＷＴ１基因表达阴性。结论：ＷＴ１基因在大多数急性白血病中表达，与白血病的病程发展可能有关，可作为检测白血病微量残留病的特异性标记。
Objective: To investigate the expression and clinical significance of WT1 gene in leukemia cells. METHODS: Four leukemia cell lines of K562, HL-60, TF-1 and U937 were detected by reverse transcription-polymerase chain reaction (RT-PCR). 45 patients with leukemia, 15 normal controls and 10 non-blood samples were used. Expression of WT1 gene in normal bone marrow cells of patients. Results: WT1 was highly expressed in K562, HL-60 and TF-1 leukemia cell lines, but not in U937 cell line. 21 of 25 acute myeloid leukemia (AML), 8 of 11 acute lymphoblastic leukemia (ALL), 2 acutely changed chronic myelogenous leukemia (CML), and 1 of 7 chronic and accelerated CML patients Positive WT1 expression. Normal bone marrow cells from 15 normal people and 10 non-hematologic patients had negative WT1 gene expression. Conclusion: The expression of WT1 gene in most acute leukemia may be related to the progression of leukemia. It can be used as a specific marker for detecting minimal residual disease in leukemia.