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用不同程度的零下低温对三种葡萄的根段进行冰冻处理,用电导法和TTC还原法检测处理组织的电解质渗出率和TTC还原率,作为冰冻损伤程度的定量比较指标。将各组处理的根段保温培养7～10天,从其外观变化鉴别死活,据以推断冰冻对根组织不可逆致伤的临界指标值和临界温度,用来比较鉴定其耐寒力强弱。结果表明:三种葡萄根的耐寒力以山葡萄最强,贝达次之,巨峰最低。且前二者的耐寒力随着冬季进程而增强,逐渐显示出其固有的耐寒遗传特性,而巨峰的变化不明显和没有规律。至春季生长期,其耐寒性都显著降低。本文还根据研究结果讨论了两种指标之间的关系和变化规律。并着重分析了次致伤低温区出现TTC还原高峰的问题。两种测定方法在研究葡萄根的耐寒力方面,表现出电导法的实用性优于TTC还原法,但TTC法在探索耐寒机理方面有其独到的灵敏性。
The root segments of three grapes were frozen with different degrees of sub-zero temperature. The electrolyte leakage rate and TTC reduction rate of the treated tissues were detected by conductivity method and TTC reduction method, which were used as a quantitative comparison indicator of the degree of freezing damage. The root segments treated in each group were incubated for 7 to 10 days, and their survival and survival were identified by their appearance changes. The critical index value and critical temperature of irreversible injury to root tissues by freezing were deduced to compare their cold tolerance strength. The results showed that the cold tolerance of the three grape roots was the strongest in the grapevine, the second in the beida and the lowest in the giant peaks. The cold resistance of the former two increased with the progress of winter, and gradually showed its inherent genetic characteristics of cold tolerance, while the change of the giant peak was not obvious and irregular. To the spring growing season, its cold resistance is significantly reduced. This article also discusses the relationship between the two indicators and the changing rules according to the research results. The problem of TTC reduction peak appeared in the low-temperature zone of secondary injury was analyzed emphatically. The two methods are better than the TTC method in studying the cold resistance of grape roots, but the TTC method has its unique sensitivity in exploring the mechanism of cold resistance.