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Bruchid is a serious insect pest of mungbean [Vigna radiata (L.) Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglü 1, from the World Vegetable Center, Asian Vegetable Research and Development Center (AVRDC). Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. To find molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR/STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus (named as Br2). Further analysis suggested that the genetic distances between these two markers and Br2 were 11.0 and 5.8 cM, respectively.
Bruquin is a serious insect pest of mungbean [Vigna radiata (L.) Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglü 1, from the World Vegetable Center, Asian Vegetable Research and Development Center (AVRDC). Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. 63. To find a molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR / STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus (named as Br2). Further analysis suggested that the genetic distance between these two markers Br2 were 11.0 and 5.8 cM, respectively.