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目的:探讨FK506结合蛋白5(FKBP5)在肝细胞癌(HCC)中的表达及临床意义。方法:用q RT-PCR和Western blot法检测30对新鲜HCC及相应癌旁组织中FKBP5 m RNA和蛋白的表达;免疫组化法检测107例HCC患者石蜡标本中FKBP5的表达,并分析其与HCC临床病理特征及预后的关系。结果:30例新鲜HCC标本中,26例(86.7%)HCC组织FKBP5 m RNA的表达水平明显高于相应癌旁组织,HCC组织中FKBP5蛋白表达量明显高于其癌旁组织。石蜡标本分析显示,FKBP5蛋白在HCC组织中的高表达率明显高于癌旁组织(63.6%vs.28.0%,P<0.001);FKBP5的表达水平与肿瘤大小(P=0.009)、肿瘤个数(P=0.018)、微血管侵犯(P=0.020)、TNM分期(P=0.009)和BCLC分期(P=0.024)有关,且FKBP5高表达患者术后总体生存率和无瘤生存率均明显低于低表达组(均P<0.001);FKBP5高表达是HCC患者术后总体生存(HR=1.992,95%CI=1.127~3.522,P=0.018)和无瘤生存(HR=1.787,95%CI=1.067~2.992,P=0.027)的独立危险因素。结论:FKBP5在HCC组织中高表达,且与患者不良临床病理特征及预后密切相关,提示其是一个潜在的HCC预后分子标志物。
Objective: To investigate the expression of FK506 binding protein 5 (FKBP5) in hepatocellular carcinoma (HCC) and its clinical significance. Methods: The expression of FKBP5 m RNA and protein in 30 pairs of fresh HCC tissues and adjacent normal tissues was detected by q RT-PCR and Western blot. The expression of FKBP5 in paraffin-embedded specimens from 107 HCC patients was detected by immunohistochemistry. Relationship between clinicopathological features and prognosis of HCC. Results: The expression of FKBP5 m RNA in 26 cases (86.7%) of HCC tissues was significantly higher than that in corresponding paracancerous tissues in 30 cases of fresh HCC specimens. The expression of FKBP5 protein in HCC tissues was significantly higher than that in paracancerous tissues. The analysis of paraffin specimens showed that the high expression rate of FKBP5 in HCC tissues was significantly higher than that in paracancer tissues (63.6% vs.28.0%, P <0.001). The expression of FKBP5 was correlated with tumor size (P = 0.009) (P = 0.018), microvascular invasion (P = 0.020), TNM staging (P = 0.009) and BCLC staging (P = 0.024). The overall survival and tumor-free survival rates of patients with high expression of FKBP5 were significantly lower (HR = 1.992, 95% CI = 1.127-3.522, P = 0.018) and tumor-free survival (HR = 1.787, 95% CI = 1.067 ~ 2.992, P = 0.027) independent risk factors. Conclusion: The high expression of FKBP5 in HCC tissues is closely related to the clinicopathological features and prognosis of patients with HCC, suggesting that it is a potential molecular marker of HCC prognosis.