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目的以实验室保藏的具有良好加工特性的20株乳酸菌为研究对象,研究它们表面疏水性、对大鼠小肠上皮细胞(IEC-6)黏附性以及对脾细胞和巨噬细胞免疫效力的影响。方法采用微生物粘着碳氢化合物法(bacteria adhesion to hydrocarbons,BATH)测定乳酸菌细胞表面疏水性;采用革兰染色法评价乳酸菌的黏附性能;采用4-甲基偶氮唑盐(MTT)染色法检测脾细胞增殖和巨噬细胞吞噬中性红能力;采用试剂盒测定脾细胞分泌肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)和巨噬细胞释放一氧化氮(NO)含量。结果菌株LV108、N4和XJH301的表面疏水性高于其他菌株(F=221.9680,P=0.0000),分别为54%、47%和44%,同时这3株菌对细胞的黏附菌数也高,均大于14个/细胞;菌株LV108可以显著诱导脾细胞增殖(F=125.0430,P=0.0000)和提高TNF-α和IL-2的释放量;菌株LV108可以显著增强巨噬细胞吞噬活性和提高NO分泌水平(F=101.0030,P=0.0000)。结论菌株LV108具有高黏附性能,可以激活免疫细胞及调节细胞因子分泌发挥一定的免疫增强作用。
Objective To investigate the effects of surface hydrophobicity, adhesion on rat intestinal epithelial cells (IEC-6), and immunological efficacy of spleen cells and macrophages, using 20 well-preserved laboratory-preserved lactic acid bacteria with good processing properties. Methods Bacterial adhesion to hydrocarbons (BATH) was used to determine the surface hydrophobicity of lactic acid bacteria cells. Gram stain was used to evaluate the adhesion of lactic acid bacteria. The spleen cells were stained with 4-methyzolamine salt (MTT) Cell proliferation and macrophages phagocytosis of neutral red; the secretion of tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2) and macrophages of nitric oxide (NO )content. Results The surface hydrophobicity of strains LV108, N4 and XJH301 were 54%, 47% and 44% higher than that of other strains (F = 221.9680, P = 0.0000) (F = 125.0430, P = 0.0000) and increased the release of TNF-α and IL-2. The strain LV108 could significantly enhance macrophage phagocytosis and increase NO Secretion level (F = 101.0030, P = 0.0000). Conclusion Strain LV108 has high adhesive properties, which can activate immune cells and regulate the secretion of cytokines to exert some immune enhancement effects.