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目的探讨芍药苷(PF)对认知功能障碍小鼠记忆功能和海马神经因子的保护作用。方法 60只小鼠随机均分为对照组(A组)、模型组(B组)、PF 50mg/kg组(C组)和PF 100mg/kg组(D组)。采用侧脑室内注射β-淀粉样蛋白诱导认知功能障碍模型,Morris水迷宫检测空间认知功能,Y型电迷宫法检测学习和记忆行为,Western blot检测海马区域的胶质原纤维酸性蛋白(GFAP)和生长相关蛋白43(GAP-43)的表达,ELISA法检测海马脑源性神经营养因子(BDNF)水平。结果与A组相比,B组训练3d后的登台潜伏期延长,站台所在象限停留时间缩短,学习次数增多,记忆次数减少,海马区域GFAP、GAP-43和BDNF水平降低(P<0.05);而PF能有效改善B组各观察指标的改变过程(P<0.05)。结论 PF能改善认知功能障碍小鼠记忆功能,同时升高海马神经因子水平。
Objective To investigate the protective effects of paeoniflorin (PF) on memory function and hippocampal neurons in mice with cognitive dysfunction. Methods Sixty mice were randomly divided into control group (group A), model group (group B), PF 50 mg / kg group (group C) and PF 100 mg / kg group (group D). The model of cognitive dysfunction induced by intracerebroventricular injection of β-amyloid was used. Morris water maze was used to detect the spatial cognitive function. The learning and memory behaviors were detected by Y-type electric maze. The expression of glial fibrillary acidic protein GFAP) and growth-associated protein 43 (GAP-43) were detected by enzyme-linked immunosorbent assay (ELISA). The levels of brain-derived neurotrophic factor (BDNF) Results Compared with group A, the incubation latency of group B after 3 days of training was prolonged, the residence time of the quadrant in platform was shortened, the number of learning times increased, the number of memory decreased, and the level of GFAP, GAP-43 and BDNF in hippocampus decreased (P <0.05) PF can effectively improve the changes of each observation index in group B (P <0.05). Conclusion PF can improve the memory function of cognitive dysfunction mice and increase the level of neurotrophic factor in hippocampus.