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分离鉴定宝华玉兰根皮乙醇总提取物(EERMZC)的挥发性成分,并考察其对小鼠成纤维细胞NCI-3T3的体外生长抑制作用。分析EERMZC的挥发性成分,用气-质联用(GC-MS)技术分析,并用峰面积归一化法测定各成分相对含量,采用MTT方法检测75,100,200,300,400μg/mL的EERMZC对NCI-3T3细胞体外生长抑制作用。气相色谱共分离出21个色谱峰,鉴定出其中14种化合物,占该挥发性成分总量的93.61%。EERMZC对NCI-3T3细胞的抑制率呈浓度依赖关系。在EERMZC的挥发性成分中,银胶菊素含量高达54.36%,其它含量较高的成分有非洲桧素(7.49%)、亚油酸乙酯(6.25%)、木香烯内酯(5.43%)等成分。EERMZC能明显抑制NCI-3T3细胞的生长。为进一步开发利用宝华玉兰药用价值提供科学依据。
The volatile components of total ethanol extract of Evaporation from Magnolia bark was isolated and identified, and its inhibitory effect on the growth of murine fibroblasts NCI-3T3 in vitro was investigated. The volatile components of EERMZC were analyzed and analyzed by gas chromatography-mass spectrometry (GC-MS). The relative content of each component was determined by peak area normalization. The inhibitory effect of EERMZC with 75,100,200,300,400μg / mL on the NCI-3T3 cells in vitro Growth inhibition. A total of 21 chromatographic peaks were identified by gas chromatography, of which 14 compounds were identified, accounting for 93.61% of the total volatile components. The inhibitory rate of EERMZC on NCI-3T3 cells in a concentration-dependent manner. In the volatile components of EERMZC, the content of guerin is as high as 54.36%. The other components with higher content are cypressin (7.49%), linoleic acid ethyl ester (6.25%), lignan lactone (5.43% ) And other ingredients. EERMZC significantly inhibited the growth of NCI-3T3 cells. To provide scientific basis for further development and utilization of Baohua Magnolia medicinal value.