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目的探讨CK19 mRNA在大肠癌患者外周血的表达及临床意义。方法应用巢式RT-PCR技术检测20例健康人、20例大肠腺瘤疾病患者和90例大肠癌患者外周血CK19 mRNA的表达。结果 健康人中CK19 mRNA不表达,大肠良性腺瘤疾病患者外周血中CK19 mRNA有1例表达,表达率为5%,大肠癌患者外周血中CK19 mRNA有53例,表达率为58.9%,CK19 mRNA表达率与肿瘤分期,瘤细胞的分化程度,肿瘤转移有显著相关性(均P<0.05)。结论 CK19 mRNA作为检测大肠癌患者外周血微转移的分子标记物具有良好的敏感度和特异性同时CK19 mRNA可作为判断大肠癌患者的预后的指标。
Objective To investigate the expression of CK19 mRNA in peripheral blood of patients with colorectal cancer and its clinical significance. Methods The expression of CK19 mRNA in peripheral blood of 20 healthy people, 20 patients with colorectal adenoma and 90 patients with colorectal cancer was detected by nested RT-PCR. Results The expression of CK19 mRNA in peripheral blood of benign adenoma patients with colorectal adenoma was 1 case, the expression rate was 5%. CK19 mRNA in peripheral blood of 53 patients with colorectal cancer was 53 cases, the expression rate was 58.9%, CK19 The mRNA expression rate was significantly correlated with tumor stage, degree of tumor cell differentiation and tumor metastasis (all P <0.05). Conclusion CK19 mRNA has good sensitivity and specificity as a molecular marker for detection of micrometastases in peripheral blood of patients with colorectal cancer. Meanwhile, CK19 mRNA may be used as an index to evaluate the prognosis of patients with colorectal cancer.