【摘 要】
：
本研究目的在于克隆人类红细胞RHD基因,构建RhD表达载体,观察其在K562细胞中的表达.从脐血网织红细胞中提取总RNA,采用逆转录聚合酶链反应扩增RHD/CE基因,扩增产物通过TA连接
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本研究目的在于克隆人类红细胞RHD基因,构建RhD表达载体,观察其在K562细胞中的表达.从脐血网织红细胞中提取总RNA,采用逆转录聚合酶链反应扩增RHD/CE基因,扩增产物通过TA连接克隆到pGEM-T质粒,采用测序方法筛选多个克隆获得RHD基因.将获得的RHD基因进一步亚克隆构建p cDNA3.1(-)表达载体,重组表达载体通过superfect试剂盒转染K562细胞,最后观察K562细胞中RHD基因的转录和表达.结果表明:成功分离克隆到RHD基因,构建的p cDNA3.1(-)重组表达载体经酶切和测
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